【摘要】 目的探讨PRDM16（PR domain containing 16）基因启动子区甲基化水平与食管癌的关联。方法运用MethyTarget方法检测40例食管癌患者的癌组织与癌旁组织中的PRDM16基因启动子区甲基化水平,构建食管癌诊断模型。结果共有25个位点甲基化水平差异有统计学意义（校正P值=0.000 5）,癌组织的甲基化水平均高于癌旁组织,两者间甲基化差值在0.13⁰.26。多因素logistics回归独立筛选出两个位点,分别为prdm16₁f₁4、prdm16₂f₆（P<0.01）。决策曲线显示prdm16₁f₁4、prdm16₂f₆联合诊断收益率高于单独诊断;ROC曲线显示prdm16₁f₁4、prdm16₂f₆单独诊断曲线下面积（AUC）分别为0.837、0.803,联合诊断AUC为0.880。结论联合prdm16₁f₁4、prdm16₂f₆位点对食管癌诊断有一定的应用价值。更多还原

【Abstract】 Objective To estimate the association of the methylation in PRDM16 gene promoter region and esophageal cancer.Methods The methylation level in promoter region of PRDM16 gene were analyzed in tissues and para cancerous tissues of40 esophageal cancer cases by MethyTarget method,the esophageal cancer diagnosis model was constructed.Results The difference of methylation levels among 25 sites were statistically significant（adj.Pvalue=0.0005）.The methylation levels of cancer tissues for above sites were higher than those of corresponding para cancerous tissues,the difference of methylation values were from 0.13 to 0.26.Multivariate logistic regression analysis identified 2 independent sites,which were prdm16₁ f₁4 and prdm16₂ f₆(P<0.01=.DCA curve showed that the yield rate of joint diagnosis of prdm16₁ f₁4 and prdm16₂ f₆ was higher than that of the single diagnosis.ROC curve showed the areas under curve（AUC）for the single diagnosis of prdm16₁ f₁4 and prdm16₂ f₆ were 0.837 and 0.803,respectively;while the AUC of joint diagnostic was 0.880.Conclusion The joint diagnosis of prdm16₁ f₁4 and prdm16₂ f₆ loci is of certain value in the diagnosis of esophageal cancer.更多还原