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以秀丽隐杆线虫为模型的玛咖功效评价
Efficacy Evaluation of Maca Using Caenorhabditis elegans as a Model
【摘要】 先对玛咖进行脱油、水提、冻干,再将所得水提物冻干品与Escherichia coli OP50菌液进行混合,稀释至终质量浓度为1,2mg/mL.以秀丽隐杆线虫(简称线虫)为模型,考察玛咖水提物对线虫的抗氧化作用:经寿命、产卵、吞咽、运动能力实验表明,玛咖水提物对线虫无明显毒性作用,且1,2mg/mL玛咖水提物均能显著延长线虫寿命;由玛咖水提物对线虫热激氧化压力诱导表明,玛咖水提物对线虫体外热激氧化压力有保护作用;通过油红O对脂肪染色表明,玛咖水提物具有一定降脂功效.
【Abstract】 Maca was extracted by oil removing,water extraction and lyophilized.The water extract was mixed with Escherichia coli OP50 broth and diluted to a final mass concentration of 1,2 mg/mL.The antioxidation effect of water extract of Maca on Caenorhabditis elegans was studied with C.elegans as a model.The experiments of lifespan,spawning,swallowing and motor ability show that the water extract of Maca has no obvious toxic effect on nematodes,and 1,2 mg/mL water extract of Maca can significantly prolong the lifespan of nematodes.The water extract of Maca has protective effect on nematode induced by oxidative stress.The oil red O staining shows that the water extract of Maca has a certain effect on lipid-lowering.
- 【文献出处】 吉林大学学报(理学版) ,Journal of Jilin University(Science Edition) , 编辑部邮箱 ,2018年02期
- 【分类号】R285;R-332
- 【下载频次】162