【摘要】 目的:探讨妊娠期糖尿病患者胎盘组织11β-HSD1和11β-HSD2表达的分子机理。方法:选择正常胎盘组织体外给予游离脂肪酸(PA)组、地塞米松(DEX)组、胰岛素(INS)组以及3种混合液(MIX)组刺激24h后,检测11β-HSD1和11β-HSD2基因和蛋白水平。结果:与基础状态相比,PA组、DEX组、INS组、MIX组胎盘组织中11β-HSD1mRNA的表达均降低(1.00±0.01、0.54±0.03、0.63±0.10、0.42±0.04、0.61±0.07,P<0.05),11β-HSD2mRNA的表达均升高(1.00±0.01、1.46±0.12、1.48±0.13、1.53±0.08、1.93±0.09,P<0.05);11β-HSD1蛋白水平表达均降低(0.99±0.01、0.43±0.05、0.18±0.02、0.71±0.09、0.54±0.06,P<0.05),11β-HSD2蛋白水平表达均提高(1.04±0.04、2.27±0.16、4.10±0.98、3.10±0.39、3.98±0.32,P<0.05)。结论:高脂血症、高皮质醇、高胰岛素可以作为独立因素调节胎盘组织中11β-HSDs的表达,妊娠期糖尿病患者高脂血症、高皮质醇血症、高胰岛素血症可能是引起11β-HSDs调控变化的分子机理。更多还原

【Abstract】 Objective:To investigate the molecular mechanism of expression of 11 beta HSD1 and 11 beta HSD2 in placental tissue of women with gestational diabetes mellitus(GDM).Methods:Normal placental tissue in vitro was treated by free fatty acids,dexamethasone,and insulin stimulation for 24 h.The levels of gene and protein expression of 11 beta HSD1 and 11 beta HSD2 were detected by real time PCR and western blot method.Results:Compared with the BASAL condition,the levels of 11 beta HSD1 mRNA expression in the placenta tissues of PA group,DEX group,INS group,and MIX group were significantly lower(the level of each group was 1.00± 0.01,0.54±0.03,0.63 ± 0.10,0.42 ± 0.04,or 0.61±0.07,respectively,P< 0.05),but the levels of 11 beta HSD2 mRNA expression in the placenta tissues of PA group,DEX group,INS group,and MIX group were significantly higher(the level of each group was 1.00±0.01,1.46±0.12,1.48±0.13,1.53±0.08,and 1.93±0.09,respectively,P<0.05).Compared with the BASAL condition,levels of 11 beta HSD1 protein expression in the placenta tissues of PA group,DEX group,INS group,and MIX group were significantly lower(the level of each group was 0.99±0.01;0.43±0.05,0.18±0.02,0.71 ± 0.09,and 0.54±0.06,respectively,P<0.05),but the levels of 11 beta HSD2 protein expression were significantly higher(the level of each group was 1.04±0.04,2.27±0.16,4.10±0.98,3.10±0.39,and 3.98±0.32,respectively,P<0.05).Conclusion:Hyperlipidemia,high cortisol or high insulin can be as single factor for regulating the expression of 11 beta HSDs in placental tissue.Hyperlipidemia,high cortisol or high insulin of women with GDM maybe the molecular mechanism of 11-beta HSDs regulation changes.更多还原