【摘要】 目的观察阿魏酸是否可调控人乳腺癌细胞系MCF-7长非编码RNA(lncRNAs)的表达水平及对细胞增殖、迁移及侵袭的影响。方法用0,1、2. 5、5、7. 5或10 mmol/L阿魏酸处理24 h后,CCK-8法检测细胞活力(n=3);软琼脂成瘤实验检测细胞体外成瘤能力; 0或2. 5 mmol/L阿魏酸处理24 h后,划痕实验及Transwell穿膜实验检测细胞增殖、迁移及侵袭能力(n=3);基因干扰和过表达技术检测雌激素受体α(ERα)是否参与阿魏酸对乳腺癌细胞的调控;反转录-实时荧光定量PCR(RT-qPCR)检测MALAT-1 mRNA表达。结果相比于对照组,阿魏酸呈浓度依赖性增加MCF-7细胞活力、增殖、成瘤、迁移和侵袭能力(P<0. 05)。干扰ERα减弱阿魏酸对MCF-7细胞生理过程的调控;过表达ERα增强阿魏酸对MB231细胞的调控。同时发现阿魏酸通过ERα调控MALAT-1的表达。结论阿魏酸通过MCF-7中ERα的介导,调控了MALAT-1的表达与乳腺癌细胞的增殖、迁移及侵袭。更多还原

【Abstract】 Objective To find out whether ferulic acid could regulate the proliferation,migration and invasion in human breast cancer cell line MCF-7. Methods After 0,1,2. 5,5,7. 5 or 10 mmol/L ferulic acid treatment for 24 h,CCK-8 assay was performed to detect cell viability and colony formation was detected by soft agar assay. After0 or 2. 5 mmol/L ferulic acid treatment for 24 h,scratch assay and transwell assay were performed to reveal migrating and invasing abilities. Gene expressing silencing and overexpression were employed to evaluate whether estrogen receptor α was involved in the regulatory role of ferulic acid. RT-qPCR analysis was performed to detect MALAT-1 levels after ferulic acid treatment. Results With growing concentration of ferulic acid,cell viability was promoted( P<0. 05). Compared to control group,ferulic acid treatment promoted proliferation,colony formation,migration and invasion in MCF-7 in a concentration dependent manner( P<0. 05). Silencing of ERα decreased the effect of ferulic acid on MCF-7; overexpression of ERα increased the effect of ferulic acid on MB231. It was also shown that ferulic acid exposure regulated MALAT-1 expressing level in a ERα dependent manner. Conclusions Ferulic acid serum regulates lncRNA MALAT-1 and the proliferation,migration and invasionin of MCF-7 cells depending on the existence of ERα receptor.更多还原