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灰葡萄孢产孢相关基因BC1G<sub>03293功能的初步研究

Function of conidiation related gene BC1G<sub>03293 in Botrytis cinerea

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【作者】 申德民谢甲涛陈桃付艳苹姜道宏程家森

【Author】 SHEN Demin;XIE Jiatao;CHEN Tao;FU Yanping;JIANG Daohong;CHENG Jiasen;Provincial Key Laboratory of Plant Pathology of Hubei Province,Huazhong Agricultural University;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University;

【机构】 华中农业大学湖北省作物病害监测和安全控制重点实验室华中农业大学农业微生物学国家重点实验室

【摘要】 据灰葡萄孢菌丝发育和菌核形成时期的RNA-seq数据,挑选出在这2个时期表达量差异较大的基因BC1G<sub>03293进行基因功能的研究。结果发现,BC1G<sub>03293在菌核发育时期表达明显上调,表达量比菌丝生长时期上调了70倍以上。该基因编码的蛋白质含有229个氨基酸,N端包含信号肽,但不含有任何已知保守结构域。通过同源重组的方法得到了BC1G<sub>03293的敲除转化子ΔBC1G<sub>03293-2和ΔBC1G<sub>03293-4,并运用农杆菌转化技术得到了互补转化子ΔBC1G<sub>03293-2-C2和ΔBC1G<sub>03293-2-C3。BC1G<sub>03293基因敲除后生长、致病及菌核形成等表型无明显变化,但敲除转化子的分生孢子产量显著下降,仅为野生型菌株B05.10分生孢子产量的45%,并且BC1G<sub>03293基因互补可使敲除转化子的产孢量得到明显恢复。结果说明BC1G<sub>03293基因与灰葡萄孢的分生孢子形成相关。

【Abstract】 According to the RNA-Seq mycelium growth and sclerotia formation stage,was selected to study in B.cinerea.The transcript level of BC1G<sub>03293 during sclerotial formation stage was higher than that during mycelium growth stage.The BC1G<sub>03293 of 229 aa signal peptide on the N end and no known conserved domain.The knockout mutantsΔBC1G<sub>03293-2 andΔBC1G<sub>03293-4 were obtained using the homologous recombination method.The complemented transformantsΔBC1G<sub>03293-2-C2 andΔBC1G<sub>03293-2-C3 were also by Agrobacterium tumefaciens-mediated transformation technique.The deletion of BC1G<sub>03293 did not affect the growth,pathogenicity and formation of sclerotia.The conidia production of the knockout mutants obviously decreased and was approximately 45% of the wild type strain.And the conidia production of the complemented transformants recovered obviously.Our research results suggested that BC1G<sub>03293 is involved the regulation of conidiation of B.cinerea.

【基金】 国家公益性行业(农业)科研专项(201303025)
  • 【文献出处】 华中农业大学学报 ,Journal of Huazhong Agricultural University , 编辑部邮箱 ,2018年01期
  • 【分类号】S432.4
  • 【被引频次】1
  • 【下载频次】255
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