【摘要】 该研究旨在筛选盐酸青藤碱与JAK/STAT信号通路的药物靶点,并对其抑菌活性与抗氧化活性进行评估.实验采用Autoduck软件筛选出药物在JAK/STAT信号通路上的药物靶标,然后用滤纸片法检测盐酸青藤碱对苏芸金杆菌和金葡萄球菌的抑菌活性,同时用DPPH法和ABTS法检测盐酸青藤碱不同溶剂提取物的抗氧化活性.结果显示:盐酸青藤碱在JAK/STAT信号通路上的药物靶点为STAT1.抑菌实验中,药物浓度为5-60mg/mL的浓度范围时,两种菌中均形成8-10mm大小不等的抑菌圈形成.且其对金葡萄球菌的抑菌效果优于苏芸金杆菌.抗氧化实验中:酸青藤碱不同溶剂提取物对[DPPH.]自由基力为:在0.1-0.4mg/mL浓度范围内,乙醇(95%)>丙酮>稀碱,在0.4-1.0mg/mL的浓度范围内,稀碱>丙酮>乙醇(95%);不同溶剂对[ABTS.]自由基的清除力为:丙酮>乙醇(95%).盐酸青藤碱有很好的抗氧化活性,但弱于VC.本实验可以为盐酸青藤碱作为新型药物开发提供实验依据,更深入探讨其潜在的药用价值提供一定的理论参考.更多还原

【Abstract】 This study aims at filtering drug targets along the signaling pathways of Sinomenine Hydrochloride and JAK/STAT,and evaluating their antibacterial and antioxidant activity. The software Autoduck was adopted to filter drug targets in the Signaling pathway of JAK/STAT,and filter paper method was used to detect antibacterial activity of Sinomenine Hydrochloride against Bacillus thuringiensis and Staphylococcus aureus;meanwhile,DPPH and ABTS methods were conducted to detect the antioxidant activity of different solvent extracts from Sinomenine Hydrochloride. The results showed that drug target of Sinomenine Hydrochloride in the signaling pathway of JAK/STAT was STAT 1. In the bacteriostasis experiment,inhibition zones varying from sizes of 8-10 mm were formed when drug concentration was in range of 5-60 mg/mL,and the bacteriostatic effect against Staphylococcus aureus was better than Bacillus thuringiensis. In the antioxidant experiment,[DPPH · ] free radical force of different solvent extracts in sinomenine hydrochloride was ethanol(95%)>acetone > dilute alkali in range of 0.1-0.4 mg/mL,and dilute alkali > acetone > ethanol(95%)in range of 0.4-1.0 mg/mL. Scavenging force of [ABTS·]free radical in different solvent was acetone > ethanol(95%). Antioxidant activity of Sinomenine Hydrochloride was relatively strong but less than VC. This experiment could provide experimental foundation for research on Sinomenine Hydrochloride used to develop new drugs,and certain theoretical reference for its potential drug value.更多还原