【摘要】 快速准确的早期诊断技术是有效防治慢性阻塞性肺疾病(COPD)、改善患者生命质量、延长其寿命、降低死亡和减少医疗费用的重要途径,也是当前医学研究中重要前沿科学问题。本研究基于患者呼出气中挥发性有机物携带大量的生理、病理信息,利用质子转移反应-飞行时间质谱仪,分析了15例患者和50例健康对照组的呼吸气中挥发性有机物的组分及浓度。初步筛选出18种COPD患者的特征性呼气化合物,其中5种化合物与前人的研究一致,可作为生物标志物应用于COPD的早期诊断。另外13种新发现的化合物,具备作为生物标志物应用于COPD早期诊断的前景,但仍需进一步的研究以证实。本研究丰富了COPD生物标志物库,对于建立非侵入性、快捷的COPD早期诊断方法具有重要支撑作用。更多还原

【Abstract】 The techniques for fast,accurate and early diagnosis of chronic obstructive pulmonary disease( COPD)are the important access to effective prevention and cure of COPD,improving the quality of their life,extending their life span,decreasing their mortality and reduction in the treatment costs. Also they are the leading-edge issues in current medicine study in the world. In the present study,based on the large amount of physio-pathological information included in the volatile organic compounds( VOCs) from the exhaled air of the patients,the proton transfer reaction time-of-flight mass spectrometry was employed to determine the components and concentrations of VOCs in inhaled and exhaled air from 15 patients of COPD and 50 healthy controls. The results showed that 5 compounds,out of the 18 compounds which were preliminarily screened as the distinctive components of the exhaled air from COPD in the present study,are consent with the results of other research,and might be used as biomarkers for the early diagnosis of COPD. Additionally,the 13 novel compounds are perspective biomarkers for the early diagnosis of COPD,but needed further deep investigation. The data of this study had enriched the biomarkers for COPD and will take an important role in the establishment of the fast-and-noninvasive early diagnosis of COPD in the future.更多还原