【摘要】 目的研究中国南海海绵Luffariellasp.的次生代谢产物及生物活性。方法利用硅胶柱色谱、C18反相柱色谱,半制备HPLC等方法对海绵提取物进行分离纯化,通过核磁共振、质谱、紫外、红外等波谱学技术,并结合文献理化数据,确定化合物的结构。对分离得到的化合物进行了NF-κB抑制活性评价实验。结果分离鉴定了6个含α、β-不饱和γ–内酯环的二倍半萜类化合物,结构确定为Hippolide H(1)、Hippolide F(2)、Hippolide G(3)、Hippolide E(4)、6Z-neomanoalide(5)、6E-neomanoalide(6)。在NF-κB抑制活性评价实验中,化合物1、4、6具有较好的NF-κB抑制活性。结论南海海绵Luffariellasp.可代谢具有NF-κB抑制活性的二倍半萜类化合物。更多还原

【Abstract】 Objective To investigate the chemical constituents and bioactivity of the South China Sea sponge Luffariellasp..Methods The compounds were isolated and purified by using silica gel column chromatography,reversed-phase C18 ODS chromatography and semi-preparative HPLC.Their structures were identified by 1 Dand 2 DNMR、MS、UV、IR and comparison with the data of literature.They were performed an NF-κB inhibitory activity evaluation test.Results Six sesterterpenoids withα、β-unsaturated-γ-lactone ring were isolated from the sponge.Their structures were identified as Hippolide H(1)、Hippolide F(2)、Hippolide G(3)、Hippolide E(4)、6 Z-neomanoalide(5)、6 E-neomanoalide(6).In the evaluation of NF-κB inhibitory activity,compounds 1,4 and6 showed good NF-κB inhibitory activity.Conclusion The South China Sea sponge Luffariella sp.could produce sesterterpenoids,some of which have good NF-κB inhibitory activity.更多还原