【摘要】 研究了海洋真菌介导邻-、间-、对-溴苯乙酮的不对称还原反应。具体考察了Rhodotorulamucilageinosa GIM 2.157对邻-、间-、对-溴苯乙酮的催化活性和对映选择性,并综合考察了反应温度、pH、反应时间和底物浓度等因素对还原产物的产率及立体选择性的影响。确定最佳反应条件:反应温度为25℃,反应体系的pH为7,反应时间为24 h,底物浓度为10 mmol/L。在最佳条件下,产率以及立体选择性均>99%。更多还原

【Abstract】 In this study, the catalytic activity and enantioselectivity of Rhodotorula mucilageinosa GIM 2.157 toward the bioreduction of o-, m-, and p-bromoacetophenone to chiral alcohols were investigated. The effects of reaction temperature, pH, reaction time, and substrate concentration on the yield and enantioselectivity of the product,(S)-1-(4-bromophenyl)ethanol, were evaluated. The determined optimum reaction conditions are as follows: 25 ℃reaction temperature, reaction system pH of 7, 24 h reaction time, and 10 mmol/L substrate concentration. Under the optimum conditions, the desired chiral alcohols were achieved with yield and ee up to > 99%.更多还原