【摘要】 目的探讨解脲脲原体(ureaplasmaurealitycum,UU)感染对大鼠睾丸组织结构及分泌功能的影响。方法 40只清洁级SD大鼠随机分为4组,每组10只,实验组A、B(7d组、14d组),对照组C、D(7d组、14d组)。实验组膀胱注射0.6mL UU4型菌株[107颜色改变单位(CCU)/mL],对照组膀胱注射等体积的UU液体培养基。分别于注射后7d、14d取各组大鼠睾丸组织于光镜和透射电镜观察镜下结构;化学发光法检测血浆睾酮和睾丸间质液睾酮含量。结果光镜观察实验组(A、B)为炎症性病变,如生精细胞数量和层次减少,炎性细胞浸润,成熟精子数量稀少等,且B组损伤性病变较A组轻,C、D对照组无明显炎症病变。透射电镜观察实验组(A、B)为生精细胞凋亡表现,如生精细胞核膜皱缩、核染色质浓缩、核破裂等,且B组凋亡情况较A组轻,C、D对照组睾丸生精细胞形态结构较为完整。血浆睾酮水平检测实验组(A、B)均低于C、D对照组(P<0.01),但A、B组间差异无统计学意义。睾丸间质液睾酮水平检测实验A组低于其余3组(P<0.01),而其余3组间差异无统计学意义。结论 UU感染大鼠睾丸组织可出现多种病理损伤进而引起分泌功能改变,进一步证实UU感染与男性不育的关系,但上述改变均可自行恢复。更多还原

【Abstract】 Objective To study the influences of ureaplasma urealitycum(UU)infection on testicular tissue structure and secretion function in rats.Methods Forty clean grade male SD rats were randomly divided into the experiment group A(at 7 dafter surgery),experiment group B(at 14 dafter surgery),control group C(at 7 d after surgery)and control group D(at 14 dafter surgery).There were 10 rats in each group.The experimental groups were injected with 0.6 mL UU4 through bladder.In the same way,the control groups were injected with the same volume of UU liquid medium.At day 7 and 14 after injection,the structures of testis of all rats were observed by light microscopy and spermatogenic cells by transmission electron microscopy.The content of testosterone in plasma and testicular fluid were detected by chemiluminescence method.Results The changes of inflammatory pathology(including the layer and amount of spermatogenic cell decreasing,inflammatory cell infiltrating and mature sperms decreasing)in the testis of group A and group B were found by light microscopy,and the inflammatory changes in group B were lighter than those in group A.The structures of testicular tissue in group C and group D were normal.The apoptosis performances of germ cell(including the cell membrane corrugated,nuclear chromatin concentration and nuclear rupture)in the testis of group A and group B were found by transmission electron microscopy,and the changes in group B were lighter than those in group A.The structures of germ cell in group C and group D were normal.The levels of plasma testosterone in group A and group B were significantly lower than that in group C and group D(P<0.01),the difference between group A and group B was not statistically significant.The testosterone level in testis interstitial fluid in group A was significantly lower than that in other groups(P<0.01),the differences between other groups were not statistical significant.ConclusionThe testicular tissue of UU infected rats can have various pathological damage and functional changes,further confirming that UU infection can cause male infertility.The pathological damage and functional changes of the testicular tissue of rats after UU infection can be gradually restored with the extension of the duration of the disease.更多还原