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耐辐射奇球菌对Cu2+和Cr6+吸附行为研究

Study of Cu2+ and Cr6+ sorption by Deinococcus radiodurans

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【作者】 郭忠忠肖方竹阳志蒋雨薇彭国文王五洲马云何淑雅

【Author】 GUO Zhong-zhong;XIAO Fang-zhu;YANG Zhi;JIANG Yu-wei;PENG Guo-wen;WANG Wu-zhou;MA Yun;HE Shu-ya;School of Public Health,University of South China;Department of Biochemistry and Molecular Biology,University of South China;School of Chemistry and Chemical Engineering,University of South China;

【机构】 南华大学公共卫生学院南华大学生物化学与分子生物学国防支撑学科实验室南华大学化学化工学院

【摘要】 本文以耐辐射奇球菌作为生物吸附剂去除溶液中的Cu2+和Cr6+,通过考察吸附时间、初始离子浓度、菌液浓度等影响吸附效果的关键因素,研究了耐辐射奇球菌对Cu2+、Cr6+的吸附作用,找出最佳吸附条件,并研究了Cu2+、Cr6+共存条件下的竞争吸附行为。采用紫外分光光度计分析耐辐射奇球菌在不同条件下对Cu2+和Cr6+的吸附作用;傅立叶红外光谱(FTIR)和扫描电镜(SEM)分析细菌吸附前后的结构和形貌变化。结果表明:耐辐射奇球菌对Cu2+和Cr6+的最佳吸附时间均为80min。对Cu2+、Cr6+吸附的最佳初始浓度分别为0.08mol·L-1、0.138mol·L-1,且随着菌液浓度的增大,耐辐射奇球菌对Cu2+、Cr6+的吸附量随之增加。当Cu2+、Cr6+共存条件下耐辐射奇球菌对Cu2+吸附量较Cr6+更大。红外光谱仪和扫描电镜结果阐释了耐辐射奇球菌对Cu2+和Cr6+的吸附行为。耐辐射奇球菌以其超强生存能力,非致病性,环境友好等优势,在重金属废水处理方面将会有很好的应用前景。

【Abstract】 In this article,Deinococcus radiodurans was used as biosorbent to remove Cu2+and Cr6+from aqueous solution.The key influencing factors including adsorption time,initial ion concentration and concentration of fluid were observed to investigate the adsorption ability of Deinococcus radiodurans.We also investigate the optimum adsorption conditions and identify the competitive adsorption behavior.UV spectrophotometer was used to analyze the adsorption efficiency in different conditions.Fourier Transform infrared spectroscopy( FTIR) and scanning electron microscopy( SEM) were used to analyze the structure and morphology changes of bacteria before and after adsorption.The results showed that the optimum adsorption time of Cu2+and Cr6+was 80 min.The optimum initial concentrations of Cu2+and Cr6+were 0. 08 mol·L-1 and 0. 138 mol·L-1,respectively.With the increased concentration of Deinococcus radiodurans,the adsorptive capacity of Cu2+and Cr6+were increased simultaneously. Competition research showed that Cu2+was adsorbed more strongly on the biomass than Cr6+. The adsorption behavior of Cu2+and Cr6+was demonstrated by Fourier Transform infrared spectroscopy and scanning electron microscopy. Deinococcus radiodurans showed potential application in wastewater treatment because of its strong survival ability,non-pathogenic and environment friendly.

【基金】 国家自然科学基金项目(11705085、51574152)资助;湖南省自然科学基金项目(2017JJ3262)资助;湖南省教育厅重点项目(17A186)资助;国家安监总局安全生产安全生产科技项目(hunan-0007-2016AQ、hunan-0010-2016AQ)资助;湖南省环境保护厅科研项目(湘财建指(2016)59号)资助;东华理工大学核资源与环境重点实验室开放基金资助项目(NRE1601)资助
  • 【文献出处】 化学研究与应用 ,Chemical Research and Application , 编辑部邮箱 ,2018年03期
  • 【分类号】O647.3
  • 【被引频次】4
  • 【下载频次】220
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