【摘要】 目的探究自噬相关基因微管相关蛋白1轻链3(LC3)和雷帕霉素靶蛋白(mTOR)在正常口腔黏膜、口腔白斑(OLK)及口腔鳞状细胞癌(OSCC)中的表达情况及相互关系,评估其作为OLK恶变早期诊断指标的临床价值。方法采用免疫组化法检测LC3B和m TOR在20例正常口腔黏膜组织、120例OLK及30例OSCC组织中的表达,回顾性分析120例OLK患者的临床资料,探讨LC3B和mTOR及其与各临床因素之间的关系。结果正常口腔黏膜、OLK和OSCC组中LC3B阳性率分别为85.0%、65.8%和33.3%(P<0.05),mTOR的阳性率分别为20.0%、48.3%和76.7%(P<0.05)。LC3B与mTOR二者间的表达具有相关性(P<0.05),且其表达都与OLK的临床类型相关(P<0.05)。结论 LC3B和mTOR可作为早期监测口腔OLK恶变的分子生物学指标。更多还原

【Abstract】 Objective This study aimed to investigate the expression and relationship of microtubule-associated protein 1 light chain 3(LC3) and mammalian target of rapamycin(mTOR) in normal oral mucosa, oral leukoplakia(OLK), and oral squamous cell carcinoma(OSCC). This work also analyzed the relationship between expression levels and clinical factors. This study evaluated the clinical value of LC3 B and mTOR as indices to determine the carcinogenic potential of OLK. Methods Immunohistochemistry was used to detect the expression of LC3 B and mTOR in 20 cases of normal oral mucosa, 120 cases of OLK, and 30 cases of OSCC. The clinical data of 120 patients with OLK were analyzed. The relationships between expression levels and clinical factors were investigated. Results In normal oral mucosa, OLK and OSCC, the positive rates of LC3 B expression were 85.0%, 65.8% and 33.3%(P<0.05), whereas the positive rates of mTOR expression were 20.0%, 48.3% and 76.7%(P<0.05). The expression levels of LC3 B and mTOR were correlated and related to clinical typing of OLK(P<0.05). Conclusion LC3 B and mTOR can be used as molecular biomarkers for early detection of OLK.更多还原