【摘要】 目的:通过分析持续丙氨酸氨基转移酶(alanine aminotransferase,ALT)正常的慢性乙型肝炎(以下简称慢乙肝)患者外泌体mi RNAs的表达情况,试图找出更好反映肝组织炎症的外泌体mi RNAs。方法:收集持续ALT正常并行肝活检的慢乙肝患者,选取肝组织炎症分级≥A2和<A2的患者各5例,提取血浆外泌体并鉴定;提取外泌体mi RNAs行高通量测序,比较两组患者的外泌体mi RNAs表达情况。结果:扫描电镜示外泌体呈囊泡状,直径30~100 nm;粒径检测示其体积峰度集中在10~100 nm之间。高通量测序共筛出591个表达有差异的外泌体mi RNAs,与对照组相比,肝组织炎症分级≥A2组有18个显著上调;6个显著下调。结论:对于肝组织炎症分级不同的持续ALT正常的慢乙肝患者,其外泌体mi RNAs的表达有差异,某些外泌体mi RNAs有望成为判断肝组织炎症的敏感生物学标志物。更多还原

【Abstract】 Objective: To investigate expression profiles of the plasma exosomal mi RNAs of the chronic hepatitis B(CHB) patients with persistently normal alamine aminotransferase(PNALT) for the first time and try to find exosomal mi RNAs which could reflect liver inflammation bett er. Methods: Five CHB patients with liver tissue inflammation grade ≥A2 of PNALT and 5 CHB patients with liver tissue inflammation grade <A2 of PNALT were enrolled and their blood samples were collected. Th e exosomes were extracted from these blood samples and measured by electron microscope to determine the extraction effect. The exosomal mi RNAs were extracted and sent for high throughput sequencing, and the expression of exosomal mi RNAs in the 2 groups of patients was analyzed. Results: Under the electron microscope, exosomes were small membranous vesicles with 30–100 nm in diameter. The peak value of particle size ranged from 10 to 100 nm. High throughput sequencing showed that there were 591 differentially expressed exosomal mi RNAs between the 2 groups. Compared with the control group, 18 exosomal mi RNAs were up-regulated and 6 exosomal mi RNAs were down-regulated in PNALT patients with the liver tissue inflammation grade≥A2. Conclusion: Exosomal mi RNAs in the CHB patients with PNALT who have the different grades of liver inflammation are differently expressed. Some of the differently expressed exosomal mi RNAs are expected to be sensitive biomarkers for timely assessment of liver inflammation in the CHB patients with PNALT.更多还原