为鉴定杨树杂交子代真实性及阐明子代群体的遗传关系,本研究以窄冠型杨树的7个杂交子代群体和亲本为试验材料,并采用SRAP分子标记技术对其进行子代真实性鉴定及遗传变异研究。从110对引物中筛选出了8对多态性高,主条带清晰且稳定的引物,对7个杂交子代群体扩增,共产生126条谱带,其中多态性带为118条,多态带比例(PPB)达93.65%;基于Pop Gen 32软件对7个杂交组合的遗传多样性分析表明,派内杂交时,黑杨派内杂交子代的遗传多样性水平低于白杨派内的遗传多样性水平;派间杂交时,白杨做母本的子代遗传多样性比黑杨做母本的遗传多样性丰富。对其中4个杂交组合的当年杂种苗的生长量、形质指标与其亲本间遗传距离进行相关性分析,结果表明,亲本间遗传距离与苗木的地径、苗高成正相关,与其分枝角度成负相关。70个杂交子代均具有父本特征带,且经鉴定全部为真杂种,本试验结果为杨树杂交亲本的筛选及杂交子代早期鉴定提供了一定的理论依据和技术参考。
【英文摘要】
To identify the authenticity of hybrid progeny and elucide the relationship of offsping population in Popular,the technology of sequence related amplified polymorphism( SRAP) was used to identify the genetic variations in 7 hybrid offspring of narrow-crowned poplar populations in this study. High primers of 8 pairs of which amplify stable main bands with clear were screened from 110 pairs of SRAP primers. A total of 126 bands were amplified from 7 hybrid progenies,including 118 polymorphic bands and the per...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
