为了解引起广西养殖龟鳖细菌性疾病的病原菌种类及其药敏特性,对2015—2016年来自广西各地的63例龟鳖病例进行病原菌的分离与纯化,并采用BIOLOG自动微生物鉴定系统结合16S r DNA测序对各病原菌进行鉴定,同时采用K-B纸片扩散法测定病原菌对头孢哌酮等27种常用抗菌药的敏感性。结果显示:被检的63例龟鳖病例中,有38例分离到以嗜水气单胞菌、肺炎克雷伯菌和变形杆菌为主的病原菌48株,25例没有分离到细菌,细菌检出率为60.3%(38/63)。各致病菌表现出不同的药敏谱和耐药谱,其中对特治星的敏感率最高,达58.3%,其次是头孢曲松和米诺环素,均为52.1%,最低的是多黏菌素B,为6.3%;对四环素的耐药率最高,达85.4%,其次是多黏菌素B,为70.8%,最低的是头孢曲松,为14.6%。说明广西龟鳖疾病仍以细菌性疾病为主,且病原复杂、存在混合感染;所检出的致病菌均存在多重耐药性。
【英文摘要】
To understand the species of pathogen and its antibiotic sensitivity in cultured turtle in Guangxi,pathogens were isolated from 63 diseased turtle samples which were collected during 2015—2016 from turtle farms in Guangxi. The strains isolated from turtle samples were identified by BIOLOG automatic microbiological assay system and 16 S rDNA sequence analysis,and their antimicrobial susceptibility was analyzed to 27 antibiotics including cefoperazone with the Kirby-Bauer disk diffusion method. The results sh...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
