【摘要】 运用实时荧光定量PCR技术,分析1只临床死亡小熊猫胃肠道13种菌群的差异性。结果显示:检测的13种菌群在小熊猫不同肠段的数量有差异,肠杆菌科(Enterobacteriaceae)在各肠段的菌群丰度差异显著(P<0.05),其菌群丰度在空肠高达9.35;白色瘤胃球菌(Ruminococcus albus)和黄色瘤胃球菌(Ruminococcus flavefaciens)的数量在直肠最多,菌群丰度分别达5.29和3.91;其他菌群的数量均在结肠最多,其中溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)、埃氏巨球形菌(Megasphaera elsdenii)和梭菌类群IV(Clostridium cluster IV)的数量在结肠中较高,菌群丰度分别为9.03、6.06和9.13;与胃相比,菌群丰度在小熊猫空肠、结肠和直肠差异显著(P<0.05)。综合分析,小熊猫的空肠、结肠和直肠对其健康生长可能起着重要的作用,肠杆菌科的细菌数量最多且在不同肠段差异显著,是今后小熊猫胃肠道菌群研究值得关注的菌群之一。更多还原

【Abstract】 In this study, real–time fluorescence quantitative PCR was used to investigate the variance of 13 bacteria communities in the different section of gastrointestinal tract(GIT) in a clinical death red panda. The investigated 13 bacteria showed variance in amount in the GIT. Enterobacteriaceae accounts for the major bacteria with its logarithm of bacterial DNA copies is 9.35. The mount of Ruminococcus albus and Ruminococcus flavefaciens was higher in the rectum, with the logarithmic values of bacterial DNA copies, 5.29 and 3.91, respectively. However, other detected bacteria remained higher number in the colon, and the logarithmic values of bacterial DNA copies of Butyrivibrio fibrisolvens, Megasphaera elsdenii, and Clostridium cluster IV were 9.03, 6.06, and 9.13, respectively. Compared with the stomach, the bacteria communities were significantly different in the jejunum, colon and rectum of the red panda(P<0.05). In conclusion, the jejunum, colon and rectum may play an important role in the growth of red panda.Enterobacteriaceae is the most abundant bacteria in the GIT and is worthy of further study.更多还原