【Objective】To raise the destruxin A production of Metarhizium anisopliae by optimizing culture conditions of liquid fermentation.【Method】Experiments were designed by response surface methodology(RSM). The key factors affecting the liquid fermentation were screened by Plackett-Burman design and central composite design.【Result】A model with high fitting degree and small error was obtained. The optimum culture condition of this model was 22.7 g·L~(–1) sucrose, 13.4 g·L~(–1) peptone and 9.70 d for fermentation....
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