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枸杞单倍体花粉败育的细胞学观察

Cytological Observation on the Pollen Abortion of Lycium barbarum Haploid from Anther Culture

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【作者】 罗青张波戴国礼赵建华曹有龙

【Author】 LUO Qing;ZHANG Bo;DAI Guo-li;ZHAO Jian-hua;CAO You-long;National Wolfberry Engineering Research Center;

【通讯作者】 曹有龙;

【机构】 国家枸杞工程技术研究中心

【摘要】 为揭示枸杞单倍体不育特性,对可育的宁杞1号和花药培养获得的单倍体的花药进行细胞学观察。结果表明:在花粉母细胞时期,宁杞1号和单倍体的花药发育没有明显差异,减数分裂后两者都顺利形成四分体。宁杞1号的四分体可以释放出小孢子,同时绒毡层细胞能够为其提供营养并发育为成熟的花粉粒。而单倍体的花药在四分体时期虽然也能释放出小孢子,但绒毡层细胞提前降解使释放出来的小孢子不能发育为成熟的花粉粒,导致单倍体花粉败育。

【Abstract】 In order to reveal the characteristics of haploidsterility,using cytological to observe the anthers of Ningqi No.1 and haploid which was obtained from anther culture.The results showed that,tetrahedron was formed in the period of pollen mother cells.There was significant difference between the material of Ningqi No.1 and haploid at the tetrad stage.Pollen grains were released from Ningqi No.1 and develop into mature,at the same time,tapetal cells began to disintegrate and disappear at last.But for haploid,microspores are released nor-mally,tapetal cells were degradated before the pollen grains ma-ture,finally pollen maturation process was aborted.

【关键词】 枸杞花药单倍体花粉败育细胞形态学
【Key words】 Lyciumantherhaploidpollen abortioncell-morphology
【基金】 宁夏回族自治区自然科学基金资助项目(NZ16097);宁夏回族自治区农业育种专项资助项目(2013NYYZ0105)
  • 【文献出处】 黑龙江农业科学 ,Heilongjiang Agricultural Sciences , 编辑部邮箱 ,2018年12期
  • 【分类号】S567.19
  • 【下载频次】195
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