In order to separate a type of antifungal protein from Bacillus subtilis Zl-2,and investigate its antifungal effects,we use the crude protein of Zl-2 to precipitate with ammonium sulfate(( NH4)2 SO4).The stability of antagonistic protein under different conditions,such as temperature,p H value,ultraviolet and protease,was investigated to understand the antifungal effects mentioned above. The antifungal activity was measured by Fusarium graminearum as an indicator. The results showed that the antifungal prot...
0引言主要由禾谷镰刀菌(Fusarium graminearum)引起的小麦赤霉病(Fusarium head blight,FHB)是世界性的流行病害,已经严重影响小麦品质,并且导致产量下降,危害人畜安全[1-2]。多年来,小麦赤霉病主要通过化学农药防治,但化学农药的大量使用导致病原菌抗药性菌株的出现,使防治难?