【摘要】 目的探讨纳米硫化镉对模式生物酿酒酵母细胞壁的损伤作用及机制。方法将目的基因——细胞壁完整性(cell wall integrity,CWI)相关基因(FKS2和PRM5)的启动子与报告基因(LacZ)相连,构建报告质粒,将其转入酿酒酵母细胞,将酿酒酵母细胞暴露于160 mg/L纳米硫化镉(nano-CdS),基于荧光白(CFW)观察酿酒酵母细胞壁损伤应答组分几丁质含量,并基于β-半乳糖苷酶活力观察酿酒酵母细胞壁完整性(CWI)报告菌株NKSY1(WT+p2052)及NKSY2(WT+p1366)细胞中FKS2和PRM5的表达,并与微米级CdS对比。结果纳米硫化镉染毒酵母细胞几丁质含量、细胞壁完整性基因FKS2及PRM5启动子活性均高于对照组(P<0.05)。结论纳米硫化镉能够损伤酿酒酵母细胞壁。更多还原

【Abstract】 Objective To understand the damage effect of cadmium sulfide nanomaterials on the cell wall of model biological Saccharomyces cerevisiae, and to illuminate the toxicological mechanism of nanomaterials. Methods Calcofluor white(CFW)was used for the quantitative analysis of yeast cell wall damage response components, chitin; Saccharomyces cerevisiae cell wall integrity(CWI) report strain NKSY2(WT+p2052) and NKSY1(WT+p1366) were employed, and the cell wall damage was determined though the β-galactosidase activity. Results Cadmium sulfide nanomaterials induced an increase of the cell wall chitin content, and the over expression of FKS2 and PRM5, thereby activating the CWI pathway. Conclusion Cadmium sulfide nanomaterials can damage the cell wall of Saccharomyces cerevisiae, and thus produce toxic effects.更多还原