【摘要】 首次利用生物酶催化法对非天然磷脂化合物磷脂酰基γ-羟基丁酸(phosphatidylbutyricacid,PB)的合成工艺进行了系统的探究。采用磷脂酶D(phospholipaseD,PLD)在有机溶媒-水两相体系中催化磷脂酰胆碱(phosphatidylcholine,PC)和γ-羟基丁酸钠(sodiumγ-hydroxybutyrate)发生磷脂酰基转移反应合成PB。实验结果表明,有机溶媒、底物摩尔比、反应温度、水相pH都对磷脂酰基γ-羟基丁酸的制备有一定的影响。确定最佳工艺为:以氯仿为有机溶媒,底物摩尔比(γ-羟基丁酸钠/PC)为90∶1,反应温度为30℃,水相pH为5.5,反应时间为6h,在此工艺条件下的磷脂酰基γ-羟基丁酸的产率为73.51%。更多还原

【Abstract】 The non-natural phosphatidylbutyric acid(PB) was synthesized for the first time using the enzyme-catalyzed method. It was synthesized by phospholipase D(PLD)-catalyzed transphosphatidylation of phosphatidylcholine(PC) with sodium γ-hydroxybutyrate in the liquid-liquid system. The operational conditions of transphosphatidylation were investigated systematically, including organic solvents, molar ratios of sodium γ-hydroxybutyrate to PC, temperature, pH, and the reaction time. The optimal reaction conditions were that: the best candidate of organic solvent was trichloromethane, the molar ratio of Sodium γ-Hydroxybutyrate to PC was 90∶1, the reaction temperature was 30℃, pH was 5.5, and the reaction time was 6 h. Under those optimal conditions, the highest yield of PB was 73.15%.更多还原