【摘要】 目的研究脾多肽对幽门螺旋杆菌脂多糖(LPS)诱导RAW264.7巨噬细胞炎症模型肿瘤坏死因子(TNF-α)、白介素(IL)-1β、IL-6及IL-17的作用。方法选取小鼠RAW264.7巨噬细胞为研究载体,分为空白组、模型组、脾多肽高、中和低浓度组,共五组,每组设3个复孔。采用幽门螺旋杆菌LPS诱导建立炎症细胞模型,予脾多肽给药干预,以37℃及5%CO2的条件下培养3 h,取上清样品备测TNF-α;再培养3 h,取上清样品备测IL-1β、IL-6与IL-17。采用酶联免疫法检测TNF-α、IL-1β、IL-6与IL-17。结果幽门螺旋杆菌脂多糖可以诱导RAW264.7巨噬细胞炎症模型。药物干预3 h后,各组TNF-α水平比较,差异有统计学意义(P<0.05),脾多肽高、中、低浓度组TNF-α水平均明显低于模型组,差异有统计学意义(P<0.05)。高浓度组TNF-α水平均明显低于中、低浓度组,差异有统计学意义(P<0.05)。药物干预6 h后,各组IL-1β、IL-6和IL-17水平比较,差异有统计学意义(P<0.05)。脾多肽高、中剂量组IL-1β、IL-6和IL-17水平均明显低于模型组,差异有统计学意义(P<0.05)。结论脾多肽能够减少幽门螺旋杆菌LPS介导的RAW264.7炎症细胞模型分泌促炎因子TNF-α、IL-1β、IL-6及IL-17,其不仅以浓度依赖降低TNF-α含量水平,还能在炎症早期抑制TNF-α,从而减轻炎症反应的发展。更多还原

【Abstract】 Objective To investigate the role of spleen polypeptides in the expression of tumour necrosis factorα(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6) and interleukin-17(IL-17) in RAW264.7 macrophage cell model induced by the lipopolysaccharide(LPS). Methods RAW264.7 cells were used as the study model and divided into5 groups, including the blank group, model group, high dose, medium dose and low dose of spleen polypeptides group,and each group had 3 repetition. Inflammatory cell model was induced by the LPS of Helicobacter pylori. Then with the situation of 37℃ and 5% CO2, each dose groups were treated with the spleen polypeptides. After 3 h of cell culture, the supernatant was collected and TNF-α was tested. Then 3 more hours later, IL-1β, IL-6 and IL-17 were tested by enzyme-linked immunesorbent assay(ELISA). Results The RAW264.7 macrophage inflammatory cell model could be successfully induced by LPS of Helicobacter pylori. After 3 h of cell culture, there were differences between the five groups in the concentration of TNF-α(P<0.05). Moreover, the concentration of TNF-α in each dose group of spleen polypeptides was obviously lower than that in the model group(P<0.05). And the concentration of TNF-α in high dose group was remarkably lower than that in the medium and low dose groups(P<0.05). After 6 h of drug treating, there were also significant differences between the five groups in the concentrations of IL-1β, IL-6 and IL-17(P<0.05). And the concentrations of IL-1β, IL-6 and IL-17 in the high and medium dose groups were significantly lower than those in the model group(P<0.05). Conclusion Spleen polypeptides can reduce the sections of TNF-α, IL-1β, IL-6 and IL-17 from the RAW264.7 inflammatory cell model induced by the LPS of Helicobacter pylori, which is not only dose-dependent on decreasing the concentrations of TNF-α, but it can also inhibit the section in the early stage of inflammation alleviating inflammatory process.更多还原