【摘要】 从田间患软腐病的不结球白菜‘福冠’植株中分离纯化得到10个菌落,对引起软腐病的关键基因——果胶盐酸裂解酶(pel)基因的保守序列进行PCR扩增,测序发现该序列与胡萝卜果胶杆菌胡萝卜亚种(Pectobacterium carotovorum subsp.carovorum)果胶裂解酶基因(Gen Bank:J03673.1)的同源性达97.7%。对该菌株进行形态及生理生化分析,发现其符合软腐病菌特征特性。接种试验发现该菌株发病症状与田间不结球白菜软腐病一致。从接种病原菌的患病组织中重新分离病原菌并进行检测,发现其与所接种的病原菌为同种病原菌。因此,所分离出的病原菌为不结球白菜软腐病病原菌,且为胡萝卜果胶杆菌胡萝卜亚种。更多还原

【Abstract】 Ten colonies were isolated and purified from soft rot diseased non-heading Chinese cabbage ‘Fuguan’plants. The pectin hydrochloric acid lyase( pel) gene was amplified through PCR and followed by nucleotide sequencing. The pel sequence shared a97.7% homology with the sequence of pectin lyase gene( Gen Bank: J03673.1) of Pectobacterium carotovorum subsp.carovorum. Morphological,physiological,and biochemical analyses of the strain was found to be consistent with the characteristics of soft rot bacterial pathogen. When the strain was inoculated into healthy non-heading Chinese cabbage,disease symptoms were produced that were consistent with the soft rot disease of non-heading cabbage in the field. The pathogens were re-isolated from the pathogen infected tissue and tested. It was found that the isolated pathogenic bacteria were the same as the inoculated pathogens. These results demonstrated that the pathogen of the non-heading cabbage soft rot disease was isolated and that it was the same as P.carotovorum subsp.carovorum.更多还原