The high density fermentation technology of Lactobacillus R8 strain was studied in this study. The main reference indexes were the density(OD600) and the utilization of carbon source(glucose). The effects of medium and agent, pH value, inoculation amount, initial sugar content, aeration mode and feeding process on the strains growth in 7.5 L stirred fermentation tank were studied, and then the experiment bacteria were optimized by 250 L pilot-scale amplification. The optimizing small-scale fermentation proc...