【摘要】 目的:探讨维生素B₃（Vit B₃）促中性粒细胞生成的可能机制。方法:取21只健康雌性SD大鼠,随机分为3组（n=7）:对照组（灌服0.9%NaCl,2 mL/d,7 d）、rhG-CSF组（皮下注射rhG-CSF,10μg/d,7 d）、Vit B₃组(灌服Vit B₃,500 mg/kg·d^-1,7 d)。检测干预前及干预后7 d中性粒细胞计数（ANC）,应用Bio-Plex悬液芯片系统检测粒细胞-巨噬细胞集落刺激因子（GM-CSF）、肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）、单核细胞趋化蛋白1（MCP-1）、巨噬细胞炎性蛋白-1α（MIP-1α）及肿瘤生长相关因子GRO/KC水平。结果:Vit B₃组和rhG-CSF组ANC较干预前及对照组显著升高（P<0.05）,但两组比较差异无统计学意义（P>0.05）。3组干预前血清各细胞因子水平比较,差异无统计学意义（P>0.05）;干预7 d后,Vit B₃组TNF-α、IL-1β、MCP-1、GRO/KC明显升高,rhG-CSF组TNF-α显著升高,Vit B₃组IL-1β、MCP-1、GRO/KC明显高于rhG-CSF组,差异均有统计学意义（均P<0.05）。结论:Vit B₃可诱导大鼠中性粒细胞生成,其机制可能与增加炎性因子TNF-α、IL-1β、MCP-1和GRO/KC分泌有关。更多还原

【Abstract】 Objective: To investigate the effect and mechanism of Vitamin B₃（Vit B₃）on granulopoiesis in normal rats. Methods: 21 healthy female SD rats were randomly divided into three groups（n=7 per group）: the control group in which the rats were given 9% NaCl via gavage,the rhG-CSF group in which the rats were given 10 μg/d rhG-CSF via subcutaneous injection for 7 days,and the Vit B₃ group in which the rats were given 500 mg/kg rhG-CSF for 7 days. The neutrophil granulocyte counts were analyzed before treatment and the 7 th day after administration. The serum cytokines such as granulocyte macrophage colony stimulating factor（GMCSF）,tumor necrosis factor（TNF）-α,interleukin（IL）-1β,monocyte chemoattractant protein-1（MCP-1）,macrophage inflammatory protein-1α（MIP-1α）and tumor growth related factors GRO/KC levels were detected by Bio-Plex suspension array system. Results: The neutrophil counts increased significantly in Vit B₃ and rhGCSF groups（P<0. 05）,but there was no significant difference between the rhG-CSF group and the Vit B₃ group（P>0. 05）. The levels of TNF-α,IL-1β,MCP-1 and GRO/KC in serum were significantly increased by Vit B₃ administration（P<0. 05）. The serum TNF-α level was increased by G-CSF treatment（P<0. 05）. The levels of IL-1β,MCP-1 and GRO/KC were markedly higher in the Vit B₃ group than those in the rhG-CSF group（P<0.05）. Conclusion: Vit B₃ could result in increasing absolute neutrophil count in healthy rats,and the mechanism might be related to the elevation of serum inflammatory factors TNF-α,IL-1β,MCP-1 and GRO/KC levels.更多还原