【Objective】Heat shock cognate protein 70 gene(HSC70)and heat shock protein 40 gene(HSP40)of goldfish were cloned,their prokaryotic expression vectors were constructed and induced by IPTG to obtain target proteins,and the biological functions of proteins HSC70 and HSP40 were surveyed to laid the foundation for revealing the mechanism of high-temperature response of goldfish.【Method】Genes HSC70 and HSP40 were amplified from total RNA isolated from goldfish liver tissues using RT-PCR. The target gene fragments...
【基金】
国家自然科学基金项目(KJZKJY13088)
【更新日期】
2018-04-19
【分类号】
S917.4
【正文快照】
0引言【研究意义】热激同源蛋白70(HSC70)是70 k D热休克蛋白家族(Hsp70)的成员之一,与细胞分化、发育密切相关,为组成型表达蛋白(Gourdon et al.,2000)。HSC70存在于所有原核及真核生物细胞中,在正常情况下稳定表达,主要参与新生肽链的折叠与组装及蛋白前体转运;在环境胁迫因?
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