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HPLC法测定康复春口服液中毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪甲苷和人参皂苷Rb₁

Determination of calycosin7-O-β-D-glucopyranoside, ononin, calycosin, formononetin, astragaloside IV, and ginsenoside Rb₁ in Kangfuchun Oral Liquid by HPLC

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【Author】 HAN Ming-yang;WANG Xue-mei;LI Yuan-yuan;MENG Xian-li;Department of Gastrointestinal Surgery, Henan Provincial People’s Hospital;Department of Traditional Chinese Medicine, Henan Provincial People’s Hospital;Department of Reproductive Center, Henan Provincial People’s Hospital;Department of Pharmacy, Henan Provincial People’s Hospital;

【机构】河南省人民医院胃肠外科；河南省人民医院中医科；河南省人民医院生殖中心；河南省人民医院药学部；

【摘要】目的建立HPLC同时测定康复春口服液中毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪甲苷和人参皂苷Rb₁的方法。方法采用Diamonsil C₁₈色谱柱（250 mm×4.6 mm,5μm）;流动相:乙腈–0.2%甲酸溶液,梯度洗脱;检测波长254 nm（0³0 min,检测毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮和芒柄花素）、210 nm（30³6 min,检测黄芪甲苷）和203 nm（36⁵0 min,检测人参皂苷Rb₁）;体积流量:0.8 mL/min;柱温:30℃;进样量为20μL。结果毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、黄芪甲苷、人参皂苷Rb₁分别在3.29⁸2.25μg/mL（r=0.999 1）、4.77¹19.25μg/mL（r=0.999 8）、4.16¹04.00μg/mL（r=0.999 2）、11.33²83.25μg/mL（r=0.999 7）、7.39¹84.75μg/mL（r=0.999 9）、2.05⁵1.25μg/mL（r=0.999 5）线性关系良好;平均加样回收率分别为97.98%、98.45%、97.38%、99.72%、98.67%、96.99%,RSD值分别0.91%、1.41%、0.78%、1.09%、1.22%、0.85%。结论方法专属性强,结果准确,重复性好,为更好地评价康复春口服液的质量提供参考。更多还原

【Abstract】 Objective To develop an HPLC method for simultaneous determination of calycosin7-O-β-D-glucopyranoside, ononin, calycosin, formononetin, astragaloside IV, and ginsenoside Rb₁ in Kangfuchun Oral Liquid. Methods The determination was carried out on Diamonsil C₁₈ chromatographic column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile-0.2% formic acid solution with gradient elution. The detection wavelengths were set at 254 nm in 0 — 30 min to determine calycosin7-O-β-D-glucopyranoside, ononin, calycosin, and formononetin, 210 nm in 30 — 36 min to determine astragaloside IV, and 203 nm in 36 — 50 min to determine ginsenoside Rb₁. The flow rate was 0.8 mL/min, temperature of column was set at 30 ℃, and volume of injection was 20 μ L. Results The linear range of calycosin7-O-β-D-glucopyranoside, ononin, calycosin, formononetin, astragaloside IV, and ginsenoside Rb₁ were 3.29 — 82.25 μ g/mL（r=0.999 1）, 4.77 — 119.25 μ g/mL（r=0.999 8）, 4.16 — 104.00 μ g/mL（r=0.999 2）, 11.33 — 283.25 μ g/mL（r=0.999 7）, 7.39 —184.75 μ g/mL（r=0.999 9）, 2.05 —51.25 μ g/mL（r=0.999 5）, respectively. The average recoveries were 97.98%), 98.45% 97.38%, 99.72%, 98.67%, and 96.99%, respectively. The corresponding RSD values were 0.91%, 1.41%, 0.78%, 1.09%, 1.22%, and 0.85%, respectively. Conclusion The method has strong specificity, accurate results, and good reproducibility which can provide a reference for quality of Kangfuchun Oral Liquid.更多还原