Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo
为探讨海藻硫酸多糖(sulfated polysaccharide from algae,SPA)及中药复方提取液(compound extracts of Chinese medicine,CECM)对H9N2亚型禽流感病毒(avian influenza virus,AIV)的作用,将SPA及CECM以3种不同加药方式作用于接种了H9N2亚型AIV的10日龄SPF鸡胚中,无菌收集鸡胚尿囊液,测定血凝效价,检测2种药液对H9N2亚型AIV的预防、治疗及直接灭活作用。结果显示,SPA及CECM的最大安全浓度分别为50和200mg/mL;3种不同加药方式下,不同浓度SPA及CECM均能极显著降低鸡胚尿囊液中H9N2亚型AIV的血凝效价(P<0.01),以直接灭活作用组的血凝效价降低幅度最大,预防作用组和治疗作用组次之。预防作用组,CECM高剂量组(200mg/mL)的抗病毒效果显著优于SPA各剂量组(12.5、25和50mg/mL)及CECM低、中剂量组(50和100 mg/mL)(P<0.05);治疗作用组,SPA高剂量组(50 mg/mL)及CECM中、高剂量组(100和200mg/mL)抗病毒效果显著优于...
【英文摘要】
To explore the effects of sulfated polysaccharide from algae(SPA)and the compound extracts of Chinese medicine(CECM)on H9 N2 subtype avian influenza virus(AIV),SPA and CECM were applied to the 10-day-old SPF chicken embryos inoculated with H9 N2 subtype AIV in 3 different ways.The chicken embryo allantoic fluid was collected aseptically,the blood coagulation titer was measured,and the prevention,treatment and direct inactivation of the two kinds of liquids to H9 N2 subtype AIV were tested.The results showed...
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