【摘要】 目的研究胃癌细胞和胃癌组织中miR-34a-5p的表达,分析其临床意义,并采用生物信息学方法进行miR-34a-5p靶基因预测和信号通路富集分析。方法采用实时荧光定量聚合酶链反应(PCR)检测miR-34a-5p在不同分化程度的胃癌细胞和正常胃黏膜上皮细胞中的表达以及在胃癌组织和癌旁组织中的表达,比较并分析其临床意义;采用miRWalk网站的生物信息学软件预测miR-34a-5p的靶基因,采用DAVID6.7软件对靶基因进行信号通路富集聚类分析。结果与正常胃黏膜上皮细胞比较,miR-34a-5p在胃癌细胞HGC-27、SGC-7901中的相对表达量未明显上调,差异均无统计学意义(P>0.05),在胃癌细胞MKN-45、MKN-28、MGC-803、BGC-823、AGS中的相对表达量均明显上调,差异有统计学意义(P<0.05或P <0.01);与癌旁组织比较,miR-34a-5p在胃癌组织中有32例表达上调[2.79(1.91,5.78)],18例表达下调[0.37(0.14,0.45)],差异有统计学意义(Z=5.821,P=0.000),提示miR-34a-5p在胃癌组织中以高表达为主;在胃癌组织中,miR-34a-5p的高表达与胃癌分化程度、淋巴结转移具有相关性(P<0.05);生物信息学分析结果提示,miR-34a-5p的靶基因富集在与肿瘤相关的28个信号通路中。结论 miR-34a-5p是胃癌的高表达分子标志物,是潜在的具有临床相关性的癌基因。更多还原

【Abstract】 Objective To observe the expression of miR-34 a-5 p in gastric cancer cells and tissues and analyze its clinical significance; to predict the target genes and analyze the signaling pathway enrichment using bioinformatics methods. Methods Real-time fluorescence quantitative PCR was used to detect the expression of miR-34 a-5 p in gastric cancer cells with different degrees of differentiation and in the normal gastric epithelial cells,and in cancerous and paracancerous gastric tissues,we made comparisons and analyzed the clinical significance. The bioinformatics software in miRWalk website was used to predict the target genes of miR-34 a-5 p and the software DAVID 6.7 was used to cluster analyze the signaling pathway enrichment of target genes. Results Compared with the normal gastric epithelial cells,the relative expression quantity of miR-34 a-5 p in gastric cancer cells HGC-27 and SGC-7901 was not significantly up-regulated,there was no statistically significant difference( P>0.05),while it all up-regulated significantly in gastric cancer cells MKN-45,MKN-28,MGC-803,BGC-823 and AGS,there were statistically significant differences( P<0.05 or P<0.01); compared with paracancerous gastric tissues,the expression of miR-34 a-5 p up-regulated in 32 cases of gastric cancer tissues[2.79( 1.91,5.78) ],down-regulated in 18 cases[0.37( 0.14,0.45) ],there was statistically significant difference( Z = 5.821,P = 0.000),which suggested that miR-34 a-5 p was highly expressed in gastric cancer tissues; in gastric cancer tissues,the high expression of miR-34 a-5 p was correlated with the degrees of differentiation and lymph node metastasis of gastric cancer( P<0.05); the analysis results of bioinformatics suggested that the target genes of miR-34 a-5 p enriched in 28 signaling pathways related to tumors.Conclusion miR-34 a-5 p is a highly expressed molecular marker in gastric cancer and is a potentially clinically relevant oncogene.更多还原