【摘要】 针对地下水抗生素抗性基因的来源和传播,结合北京再生水入渗现场四环素类抗生素抗性基因的分布特征,以p UC18质粒作为载体,分别导入tet B、tet M、tet O和tet W基因片段作为模式抗性基因,分析了游离态四环素类抗生素抗性基因在土壤中的吸附规律。结果表明,对于四环素类抗生素抗性基因,不同目的片段对其在包气带土壤介质中的平衡吸附量没有影响,而底泥对携带tet O和tet W目的片段的质粒吸附量相对较高。更多还原

【Abstract】 In this study,tetracyclines antibiotic resistance genes using p UC18 plasmids( pattern resistant genes were tetB,tetM,tetO and tetW) as carriers were considered as the targeted pollutants,and the adsorption and transformation characteristics from a reclaimed water infiltration site in Beijing were analyzed in the batch and column experiments. The results revealed that the adsorption of the plasmids with different target genes was similar in the vadose zone,whereas the adsorptions of tetO and tetW genes were higher in sediment soil.更多还原