【摘要】 目的:探讨染料木素磺酸钠(GSS)对大鼠脑缺血再灌注损伤后脑组织中基质金属蛋白酶-3(MMP-3)、金属蛋白酶-9(MMP-9)表达的影响。方法:采用大鼠大脑中动脉栓塞(MCAO)动物模型,观察脑组织水肿程度及脑组织MMP-3和MMP-9的mRNA及蛋白表达水平。结果:GSS治疗组脑水肿减轻,GSS处理后6,12,24 h MMP-3和MMP-9 mRNA较模型组水平降低,以12和24 h明显。I/R模型组再灌注24 h后缺血半暗带区脑组织MMP-3和MMP-9蛋白表达明显升高,GSS处理后明显降低两种蛋白表达水平。结论:染料木素磺酸钠对脑缺血再灌注损伤的保护作用机制之一可能与抑制了MMP-3及MMP-9蛋白表达有关。更多还原

【Abstract】 Objective: To investigate the inhibition effect of genistein sodium sulfonate( GSS) on marixmetallo proteinase-3 and 9 induced by cerebral ischemia reperfusion in rats. Methods: A rat cerebral ischemia/reperfusion injury was made through embolization of the middle cerebral artery. The degree of edema in brain tissue was observed. The mRNA and protein expression levels of MMP-3 and MMP-9 on half injury brain tissue were detected by Real time-PCR and western blot.Results: Brain edema in the GSS treatment group was reduced compared with the model group. The mRNA levels of the two groups at 6,12,and 24 h after GSS treatment were lower than those in the model group,and were evident at 12 and24 h. The expression of MMP-3 and MMP-9 protein in the ischemic penumbra was significantly increased in I/R model group 24 h after reperfusion,which were significantly decreased after GSS treatment. Conclusions: Inhibiting the expression and activity of MMP-3 and MMP-9 may be one of protective mechanisms of GSS on cerebral ischemic/reperfusion injury rats.更多还原