【摘要】 在过去几十年中,大量分子被研究作为模板应用于分子印迹技术从而实现分离、催化、药物输送等,然而其中对于大分子的研究仍是较少的。近年来,研究者将分子印迹技术与凝胶光子晶体结合所得分子印迹凝胶光子晶体(MIPC)方法已成为一种新型大分子检测手段,其印迹位点多少与三维孔道结构完整性是影响响应性的两个关键因素,因而选择合适的单体与适当的胶体晶体制备方法至关重要。采用一种新的功能单体衣康酸,单分子中具有两个活性羧基基团,可同时与印迹分子结合形成特异性印迹位点,同时借助"三明治"结构控制前驱液的填充有效制备反蛋白石结构,得到可无标记比色检测四环素的分子印迹凝胶光子晶体膜.以未添加印迹分子的非印迹薄膜作为对照,将洗脱胶晶模板与印迹分子的薄膜及对照薄膜分别浸入四环素检测液中,当四环素浓度由10-9~10-4 mol/L变化时,印迹薄膜的Bragg衍射峰位移达到111nm,其宏观颜色由紫色变为黄绿色,颜色变化可由肉眼明显识别,从而实现半定量快速检测,而无印迹薄膜在相同浓度变化时Bragg衍射峰仅发生微小变化,宏观颜色变化不明显.相较于传统检测手段耗时、费力、仪器操作复杂等问题,采用分子印迹凝胶光子晶体方法灵敏、快速、操作简便,为药物分子检测提供了新的思路,有望应用于临床和食品快速检测。更多还原

【Abstract】 A colorimetric molecular imprinted photonic crystal sensor(MIPC)prepared based on the combination of a colloidal crystal templating method and a molecular imprinting technique has become a new detection method for macromolecules.The number of imprinted sites and the structural integrity of three-dimensional channels are two key factors influencing the responsiveness.In this paper,a new functional monomer,itaconic acid which has two active carboxyl groups in the single molecule,was adopted to form a highly sensitive colorimetric MIPC film for the detection of trace tetracycline with the aid of the "sandwich" structure to control the filling of the precursor fluid.After removal of the colloidal crystal template and the molecularly imprinted template,the final inverse opal MIPC film was obtained.The Bragg diffraction peak displacement of the film reached 111 nm,and its macroscopic color changed from purple to yellow-green when the concentration of tetracycline in the range of 10-9 mol/L to 10-4 mol/L which can be clearly identified by naked eyes.In this case,a sensitive,visible and convenient sensor was obtained,the developed method was expected to be applied to clinical and food testing.更多还原