【摘要】 目的研究粒细胞集落刺激因子（GCSF）对急性心肌梗死大鼠炎症因子及相关凋亡指标的影响。方法按照体重将SD雄性大鼠随机分为4组,每组各15只:假手术组、模型组、GCSF小剂量递增组（GCSF-L-I）与GCSF大剂量组（GCSF-H）。建立心肌梗死动物模型后,GCSF-L-I组大鼠给予GCSF初始剂量5μg·kg^-1,随后每天增加剂量5μg·kg^-1,连续给药1周,总剂量达140μg·kg^-1;GCSF-H组大鼠给予GCSF 20μg·kg^-1·d^-1,连续给药1周,总剂量亦达140μg·kg^-1;假手术组、模型组给予等量0.9%Na Cl。检测各组大鼠的血液细胞学指标、炎症因子指标及心功能指标,建模术后第2周通过免疫印迹法测定各组大鼠相关细胞凋亡因子B细胞淋巴瘤-2（Bcl-2）及B淋巴瘤2-基因相关X蛋白（Bax）表达。结果建模2周,假手术组、模型组、GCSF-L-I组和GCSF-H组的白细胞数量分别为（6.15±0.28）,（6.35±0.68）,（8.20±1.51）,（7.46±2.43）×10⁹/L,模型组与假手术组比较,差异无统计学意义（P>0.05）;GCSF-L-I组和GCSF-H组与模型组比较,差异均有统计学意义（均P<0.05）。假手术组、模型组、GCSF-L-I组和GCSF-H组的超敏C反应蛋白分别为（1.25±0.23）,（4.16±0.71）,（2.00±0.69）,（2.12±0.72）mg·L^-1,模型组、CSF-L-I组和GCSF-H组与假手术组比较,差异均有统计学意义（均P<0.05）;GCSF-L-I组和GCSF-H组与模型组比较,差异均有统计学意义（均P<0.05）。假手术组、模型组、GCSF-L-I组和GCSF-H组的左室射血分数分别为（70.16±2.95）%,（43.36±3.10）%,（65.45±2.23）%,（56.27±2.36）%,模型组与假手术组比较,差异有统计学意义（P<0.05）;GCSF-L-I组和GCSF-H组与模型组比较,差异均有统计学意义（均P<0.05）;GCSF-L-I组明显高于GCSF-H组,差异有统计学意义（P<0.05）。结论 GCSF通过调控炎症反应、抑制凋亡,明显改善了大鼠心梗后心功能,且GCSF小剂量递增干预效果更明显。更多还原

【Abstract】 Objective To study the effect of granulocyte colony-stimulating factor（GCSF）on inflammatory factors and related apoptosis indexes in rats with acute myocardial infarction（AMI）.Methods SD rats were randomly divided into 4 groups:sham operation group,model group,low-dose GCSF increasing（GCSF-L-I）group and high-dose GCSF（GCSF-H）group.After the AMI model established,GCSF-L-I group,after coronary artery ligation,intraperitoneally injected GCSF,initial dose 5μg·kg^-1,then a daily increase of 5μg·kg^-1,continued the medication for a week,the total dose of GCSF was 140μg·kg^-1;GCSF-H group,after coronary artery ligation,intraperitoneally injected GCSF 20μg·kg^-1·d^-1,continued the medication for a week,the total dose of GCSF was also140μg·kg^-1;sham operation group and model group were given the same amount of 0.9%sodium chloride injection.The indexes of blood cytology,inflammatory factors and cardiac function were detected at 1 and 2 weeks after modeling in each group.At the time of 2 weeks after modeling,the expression of apoptosis related factors B-cell lymphoma-2（Bcl-2）and BCL2-associated X protein（Bax）were detected by Western blot.Results Two weeks after modeling,the number of white blood cells in sham operation group,model group,GCSF-L-I group and GCSF-H group were respectively（6.15±0.28）,（6.35±0.68）,（8.20±1.51）,（7.46±2.43）×10⁹·L^-1;compared with the sham operation group,there was no significant change in the model group（P>0.05）;compared with the model group,the GCSF-L-I group and the GCSF-H group were significantly increased（all P<0.05）.The high sensitive C-reactive protein levels in the 4 groups were respectively（1.25±0.23）,（4.16±0.71）,（2.00±0.69）,（2.12±0.72）mg·L^-1.The left ventricular ejection fraction in the 4 groups were respectively（70.16±2.95）%,（43.36±3.10）%,（65.45±2.23）%,（56.27±2.36）%.Compared the sham operation group with the model group,the difference of the factors were significant（all P<0.05）.Compared the model group with the GCSF-L-I group and the GCSF-H group were all significant（all P<0.05）.GCSF-L-I group was significantly higher than that in GCSF-H group（P<0.05）.Conclusion GCSF significantly improved the cardiac function of rats after myocardial infarction by regulating inflammatory reaction and inhibiting apoptosis,and the effect of low dose increasing of GCSF was more obvious.更多还原