Objective To modify current acetylcholinesterase histochemical staining procedure for rapid pathological diagnosis of Hirschsprung's disease during surgery. Methods Based on conventional Karnovsky Roots method, new procedure increased the concentration of iodinated acetylthiocholine by 10 times, allowing a faster tissue infiltration and shorter incubation time. After incubation, diaminobenzidine(DAB) was applied to stabilize positive staining and increase stain contrast for better slide viewing under micros...