Objective To explore the spermatogenesis after hibernation in Chinese soft-shelled turtles. Methods Testicular tissues were collected in September, December, and March of next year. The spermatogenesis was analyzed through comparative morphological studies and evaluation of creatine kinase expression and localization. Results There were spermatogenesis and sperm release in the seminiferous tubules after hibernation, which is different from the traditional view. Moreover, as a key gene of energy metabolism, ...