【摘要】 目的探讨乙型肝炎病毒感染引起的慢加急性肝衰竭患者血IFN-γ基因多态性。方法采用单核苷酸多态性(SNP)技术检测51例ACLF患者和50例健康人血IFN-γ基因内含子+874位点T/A和+2109位点A/G单核苷酸多态性。结果 ACLF患者+874位点TA+AA基因型频率(54.9%)显著高于健康人(24.0%),A等位基因频率(38.2%)显著高于健康人(22.0%,P<0.05);ACLF组+2109位点AG+GG型基因型频率(51.0%)显著高于健康人(26.0%),G等位基因(32.4%)显著高于健康人(16.0%,P<0.05);在3 m末,28例生存与23例死亡的ACLF患者+874、+2109位点等位基因和基因型频率分布无显著性差异(P>0.05)。结论 IFN-γ基因+874位点A等位基因和基因型、+2109位点G等位基因和基因型是ACLF的遗传易感基因。更多还原

【Abstract】 Objective To investigate blood IFN-γ gene polymorphism in patients with hepatitis B-induced acute-on-chronic liver failure(ACLF). Method Single nucleotide polymorphism(SNP) technique was applied to detect blood single nucleotide polymorphism of IFN-γ gene +874 T/A and +2109 A/G in 51 patients with ACLF and 50 healthy controls. Results The frequency of +874 locus TA+AA genotype in patients with ACLF was54.9%,much higher than 24.0% in the control(P<0.05),and A allel was 38.2%,also much higher than 22.0% in the control(P<0.05);the frequency of +2109 locus AG+GG genotype in patients with ACLF was 51.0%,much higher than 26.0% in the control(P<0.05),and G allel was 32.4%,much higher than 16.0% in the control(P<0.05);there was no significant difference as respect to the frequencies of +874 and +2109 allele and genotype between28 survivals and 23 dead(P>0.05) at the end of 3 month follow-up. Conclusion IFN-γ gene +874 locus A allel and its genotype and +2109 locus G allel and its genotype might be the genetic susceptibility genes for the occurrence of ACLF.更多还原