【摘要】 为降低铁皮石斛组织培养成本、简化组织培养程序,以铁皮石斛原球茎为试验材料,采用开放式组织培养方法研究MS固体培养基添加不同浓度代森锰锌和链霉素对铁皮石斛原球茎组织增殖、分化、生根及抑菌效果的影响。结果表明:在培养基中,添加0.3g/L代森锰锌+1.5g/L链霉素有利于铁皮石斛原球茎增殖;添加0.3~0.5g/L代森锰锌+1.5g/L链霉素的抑菌效果较好,并能促进分化幼苗生长;添加0.5g/L代森锰锌+1.5~2.0g/L链霉素有利于再生植株生根。更多还原

【Abstract】 The Dendrobium officinale protocorms were cultured on MS solid media added with different concentration of Mancozeb and Streptomycin to study antibacterial effects of antibacterial agents on proliferation,differentiation and rooting of D.officinale protocorms and to provide a reference for reducing tissue culture cost of D.officinale and simplifying tissue culture process.Result:MS solid medium added with 0.3 g/L Mancozeb + 1.5 g/L Streptomycin is beneficial to proliferation of D.officinale protocorms.MS solid medium added with 0.3~0.5 g/L Mancozeb + 1.5 g/L Streptomycin has good antibacterial effect and promotes growth of differentiated plantlets.MS solid medium added with0.5 g/L Mancozeb + 1.5~2.0 g/L Streptomycin is beneficial to rooting of plantlets.更多还原