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车前叶蓝蓟籽油提取方法和溶剂的优化

Extraction Method and Solvent Optimization of Echium plantagineum Seed Oil

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【作者】 江喻罗世家邓志军刘欢邓仕明

【Author】 Jiang Yu;Luo Shijia;Deng Zhijun;Liu Huan;Deng Shiming;Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei University for Nationalities;College of Horticulture Forestry, Hubei University for Nationality;

【通讯作者】 邓仕明;

【机构】 湖北民族学院生物资源保护与利用湖北省重点实验室湖北民族学院林学园艺学院

【摘要】 本研究应用了加压液体萃取(pressurized liquid extraction, PLE)、微波辅助萃取(microwave assisted extraction, MAE)和超声辅助萃取(ultrasound assisted extraction, UAE)三种现代提取技术对车前叶蓝蓟籽油进行提取,并对乙酸乙酯、乙醇、水及其混合液作为溶剂的萃取条件进行了优化,以实现作为正己烷的替代绿色萃取溶剂。本研究表明,乙醇是一种作为车前叶蓝蓟籽油提取的非常有优势的绿色提取溶剂。PLE提取产量最高(32.1%),但温度也相对较高,可能导致部分不稳定化合物热降解。UAE (29.8%)的主要特点是在温和条件下进行的,低温(60℃)允许使用UAE作为热不稳定化合物的提取。另一方面,无论是开放系统(22.6%)还是封闭系统(24.9%),MAE都显示出所有测试技术的最低产油量。然而,MAE的优势主要是节省时间,并且可以每隔几分钟可重复进行一次快速有效的萃取。

【Abstract】 Three modern extraction technologies, namely pressurized fluid extraction(PLE), microwave-assisted extraction(MAE) and ultrasonic assisted extraction(UAE), were used in this study for seed oil extraction of Echium plantagineum L.. In order to realize the alternative green extraction solvent as n-hexane, the extraction conditions of ethyl acetate, ethanol, water, and their mixtures as solvents were optimized. The results showed that ethanol is a very advantageous green extraction solvent for the extraction of seed oil of Echium plantagineum. The highest yield of PLE extraction(32.1%), but the relatively high temperature, may lead to thermal degradation of some unstable compounds. The main feature of UAE(29.8%) was performed under mild conditions, and low temperature(60℃) allowed the use of UAE as the extraction of thermally unstable compounds. On the other hand,MAE showed the lowest production of all test technologies, whether it was open system(22.6%) or close system(24.9%). However, the main advantage of MAE would be time-saving and could be repeated once every few minutes for a quick and efficient extraction.

【基金】 生物资源保护与利用湖北省重点实验室项目(PKLHB1722);国家自然科学基金项目(31460203)共同资助
  • 【文献出处】 分子植物育种 ,Molecular Plant Breeding , 编辑部邮箱 ,2018年23期
  • 【分类号】TS224
  • 【下载频次】133
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