【摘要】 为丰富太子参分子标记库、开发太子参SSR标记、寻找有效的太子参种源鉴定方法。本研究运用MISA、Primer3等分子生物学分析工具,从太子参转录组中搜索获得11 297个SSR位点,其出现频率为8.87%,分布距离为平均每10 kb有1.47个SSR位点,其中主要重复类型是单核苷酸重复;11 297个SSR位点中不同核苷酸基序类型的SSR有259种,主要以单核苷酸重复基序A/T为主,其次是三核苷酸重复基序AAT/ATT。在此基础上设计、筛选得到16 821对特异性引物,其中针对高多态性SSR序列的引物有8 706对,随机挑选其中的20对引物对来源于贵州、江苏、福建的6个太子参样品DNA进行PCR扩增,其扩增成功率达70%～80%。太子参转录组SSR分布密度大、类型丰富、多态性潜能高、通用性好,可为开发太子参功能性标记提供前期基础,在太子参种质资源评价和优质种源筛选等方面具有极大的开发价值和良好的应用前景。更多还原

【Abstract】 In order to enrich the molecular marker library, develop SSR markers and find effective provenance identification methods of Pseudostellaria heterophylla, 11 297 SSR loci with the occurrence frequency of 8.87%were obtained from transcriptome of P. heterophylla by using MISA, Primer3 and other molecular biological analysis tools. The distribution distance of the SSRs was about 1.47 SSRs per 10 kb ESTs, among which the main repeat type was single nucleotide repeat. 259 SSR types of different motifs in nucleotide sequences were identified in 11 297 SSRs, in which the single nucleotide repeat motifs of A/T was the main type, followed by trinucleotide repeat motifs of AAT/ATT. 16 821 pairs of specific primers were designed and screened on this basis, including8 706 pairs of primers for high polymorphic SSR sequences, 20 pairs of primers were randomly selected for PCR amplification on 6 samples of P. heterophylla from Guizhou, Jiangsu and Fujian. The successful amplification rate was up to 70%～80%. The SSR from P. heterophylla transcriptome was characterized with distribution density, rich types, high potential polymorphism and good versatility, which might provide a basis for the development of functional markers of P. heterophylla and might be of great value in exploiting and application on evaluation and selection of germplasm resources of P. heterophylla.更多还原