自然杂交实生苗选育是获得蓝莓优良品系或新品种的重要手段之一,但是父本未知是该种选育方法的最大缺点,所以利用分子生物学技术从授粉群体中鉴定其父本来源并建立相应的DNA指纹图谱,对于蓝莓优良品系或新品种的利用与保护具有重要价值。本研究利用EST-SSR分子标记技术对优良品系的父本进行了鉴定,并构建了相应的DNA指纹图谱。结果表明,从40对EST-SSR引物中筛选出10对条带清晰、多态性较高的引物组合,其中检测出等位基因23个,且每个位点的等位基因数在2~3之间,平均每个位点包含2.3个等位基因;另外,平均Shannon多样性指数为0.700 3。基于遗传相似系数,利用(unweighted pair-group method with arithmetic means,UPGMA)非加权类平均法对8个自然杂交蓝莓优良品系与其待选父本的亲缘关系进行了分析,明确了父本来源并获得杂交系谱:即SMN-72(爱国者×斯巴坦);SMN-86(日出×艾克塔);SMN-234(蓝丰×迪克西);SMN-291(爱国者×大粒蓝金);SMN-339(爱国者×斯巴坦);SMN-350(陶柔×塞拉);SMN-460(柳叶×陶柔);SMN-...
【英文摘要】
Breeding of natural hybrid seedlings is one of the important ways to obtain excellent blueberry strains or new varieties, but the unknown male is the biggest drawback of breeding method, so the use of molecular biology techniques to identify the paternal origin from pollination groups and the establishment of DNA fingerprint have the great value for the utilization and protection of blueberry clones or new varieties. In this study, the male parent of fine strain was identified by EST-SSR molecular marker, a...
Male Parent Identification of Blueberry Excellent Hybridization Strains andConstruction of DNA FingerprintXu Guohui2,3Gao Xiongmei1,3Zhao Lina1,3Wang Hexin2,3Hou Yilong1Yan Dongling1,3Lv Jiekai1FengNing1Liu Guoling3Xiao Wei41 School of Life Science and Te
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
