【摘要】 探讨复方树莓籽粉水溶液对急性放射性损伤小鼠骨髓细胞的影响及防护作用。建立小鼠急性放射性损伤模型,设立空白对照组、模型对照组及不同浓度复方树莓籽粉溶液处理组(低、中、高剂量组),观察辐照后各组小鼠骨髓细胞有核细胞数、微核率、细胞周期及线粒体膜电位的变化情况。实验结果表明,与空白对照组比较,模型对照组变化均非常显著(p<0.01)。与模型对照组比较,复方树莓籽粉中、高剂量组有核细胞数分别升高78.2%(p<0.01)、116.9%(p<0.01);高剂量组微核率减少27.3%(p<0.01);中剂量组细胞周期G1期、S期,高剂量组S期细胞比例升高(p<0.05),高剂量组G1期细胞比例显著升高(p<0.01);中、高剂量组骨髓细胞线粒体膜电位显著升高(p<0.01)。实验结果提示,复方树莓籽粉能升高骨髓细胞数,降低骨髓细胞微核率,升高骨髓细胞线粒体膜电位,减少骨髓细胞周期阻滞,抑制辐射损伤骨髓细胞的细胞凋亡,可能对辐照导致的骨髓细胞损伤有防护作用。更多还原

【Abstract】 This study explores the protective effect of compound raspberry seed powder on the bone marrow cells of acute radiation-injured mice. In the experiment, an acute radiation injury model was generated in mice. A blank control group, a model control group, and treatment groups of different concentrations of compound raspberry seed powder solution(low/medium/high dose group) were established. After irradiation, the changes in nucleus cells, micronucleus rate, cell cycle, and mitochondrial membrane potential of mice bone marrow cells were measured. Compared with the blank control group, the model control group and treatment groups were significantly different(p<0.01).Compared with the model control group, the number of nucleated cells increased by 78.2%(p<0.01) and 116.9%(p<0.01), respectively, in the medium, and high dose groups, and the micronucleus rate in the high dose group decreased by 27.3%(p<0.01). The proportion of cells in the G1 phase and S phase in the medium dose group and in the S phase of high dose group was increased(p<0.05). The proportion of cells in the G1 phase of the high dose group increased significantly(p<0.01). The mitochondrial membrane potential of bone marrow cells in the medium and high dose groups was significantly increased(p<0.01). The results indicated that compound raspberry seed powder increased the number of bone marrow cells, reduced the micronucleus rate, increased the mitochondrial membrane potential, reduced cell cycle arrest, and inhibited the induction of apoptosis by irradiation damage in bone marrow cells, which suggested that compound raspberry seed powder may play an important role in the protection of bone marrow cells injured by irradiation.更多还原