【摘要】 采用⁶⁰Co γ-射线辐射结合抗性筛选技术,对替考拉宁产生菌替考游动放线菌FIM-16的孢子进行诱变处理,以替考拉宁自身抗性作为选择压力,并通过摇瓶发酵对抗性基因突变株进行筛选。结果获得一株摇瓶发酵水平较出发菌株FIM-16提高两倍的高产菌FIM-16-58,且遗传性状相对稳定,菌种传3代发酵水平无明显影响,菌种斜面在4°C稳定保藏两个月;其在1 T发酵罐上发酵,添加0.1%的玉米油可有效抑制泡沫的产生,替考拉宁产量达5.0 g/L以上。更多还原

【Abstract】 To screen high-yield teicoplanin-producing strain, ⁶⁰Co γ-ray irradiated spore suspension of the strain Actinoplanes teichomyceticus FIM-16 was spread on regenerating plate containing lethal concentration of teicoplanin. Teicoplanin-resistant mutants were picked and screened by shake-flask fermentation. Strain FIM-16-58 showed two times higher teicoplanin yield than the original strain, and demonstrated higher hereditary stability. There was no obvious effect on the fermentation level of the strain over three generations, and its slope was stable at 4 °C for two months. During scale-up experiment in the 1 T fermenter, foam was suppressed effectively by adding 0.1% corn oil, and the yield of teicoplanin reached over 5.0 g/L. Therefore, the high-yield strain laid the foundation of its industrial production and fermentation.更多还原