【摘要】 随着现代医学以及纳米科技的发展,聚乙二醇修饰的纳米探针在分子成像领域引起了广泛关注,在胃肠道疾病的诊断和预后中发挥了重要作用。本研究构建了基于过渡金属氧化物的聚乙二醇修饰氧化钨纳米探针(PEG-WO_3-x),用于胃肠道疾病CT成像分析。首先通过高温溶剂热方法合成氧化钨纳米探针,用聚乙二醇修饰以提高其在水中的分散性及稳定性。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X-射线光电子能谱（XPS）和红外光谱等对合成的PEG-WO_3-x进行了表征,通过组织病理学方法评价其在大鼠体内的毒性。结果表明,PEG-WO_3-x具有较低的体内毒性。考察了PEG-WO_3-x体外以及大鼠体内的螺旋CT成像效果,结果表明,PEG-WO_3-x具有良好的胃肠道CT成像能力。PEG-WO_3-x在螺旋CT对胃肠道疾病的诊断中具有良好的应用前景。更多还原

【Abstract】 Polyethyleneglycol（ PEG）-modified（ PEGylated） nanoprobes have attracted a great deal of interest in the area of molecular imaging area along with the rapid development of modern medicine. These nanoprobes play an important role especially in the diagnosis and prognosis of gastric diseases. The transition metal oxides as gastric contrast agents based on PEGylated WO_3-x nanoprobes( PEG-WO_3-x) were designed and synthesized. The nanoprobes ewere synthesized via a high-temperature solvent thermal method,and then modified with PEG. These PEGylated nanoprobes exhibited excellent aqueous stability. Besides evaluating the characteristics of PEG-WO_3-x,in vivo,toxicological studies were carried out with histopathology. Otherwise,as a contrast agent,MDCT imaging was illustrated via both in vitro and in vivo assessment. The experimental results indicated that the prepared PEG-WO_3-x showed great potential for further MDCT imaging in detection of gastric diseases.更多还原