【摘要】 制备了表面电性可控的氨基化SiO₂@Fe₃O₄磁性复合微球,采用激光刻蚀和热压键合的方法制作了聚甲基丙烯酸甲酯（PMMA）材质的DNA固相萃取芯片,将磁珠灌注于芯片通道中,借助永磁铁固定并控制磁珠,将磁珠芯片应用于人类全血中的基因组DNA提取,优化了提取实验条件,并对提取产物进行凝胶电泳和PCR分析。实验结果表明,磁珠微流控芯片成功地从全血中提取出纯度较高的基因组DNA,提取效率约35%,提取液的凝胶电泳条带与商品化试剂盒提取的基因组DNA一致,提取液可用于进一步的PCR反应。更多还原

【Abstract】 Self-made surface charge switching amino-SiO₂@Fe₃O₄ magnetic composite microspheres were applied to microfluidic chip,and DNA solid phase extraction chip of polymethyl methacrylate（ PMMA） was fabricated by laser etching and hot pressing bonding. The magnetic beads that could be controlled by permanent magnet were injected into the chip channel to obtain a magnetic bead chip for genomic DNA extraction in human whole blood. Under the optimized extraction conditions（ with 80 μg beads,flow rate for adsorption,elution and desorption were 10,5 and 20 μL/min,respectively）,the extracted products were subjected to gel electrophoresis and PCR test. The results showed that the genomic DNA with high purity was extracted from the whole blood by magnetic bead microfluidic chip. The extraction efficiency was about 35%.The gel electrophoresis band of the extract was consistent with the genomic DNA extracted by commercial reagent kit. The extract solution could be used for further PCR reaction.更多还原