【摘要】 单个纳米孔电流脉冲法是一种新型、快速、简便的检测手段,有望实现单分子核酸测序和生物传感。由于通常条件下分子穿孔导致的电流脉冲时间短,电流水平低,对脉冲检测系统提出了很高的要求。本研究基于自行组建的单个纳米孔电流脉冲检测系统,以单个α-溶血素(α-HL)纳米孔为界面,探究了鱼精蛋白在调控ss DNA电流脉冲中的作用。结果表明,此系统不仅能够分别观测带正电的鱼精蛋白和带负电的ssDNA探针的电流脉冲信号,而且加入鱼精蛋白,可使ss DNA电流脉冲的幅度及停留时间均显著增加。本研究为基于分子相互作用提高电流脉冲的分辨能力提供了途径。更多还原

【Abstract】 Single nanopore current pulse method is a new,rapid and simple detection method,which is promising for single-molecule DNA sequencing and bio-sensing. Due to the short duration and the low current amplitude of the pulses caused by molecular translocation under normal conditions,pulse detection system with fast response and high sensitivity is required. In this work,based on a lab-established pulse detection system,the effect of protamine in the regulation of single-stranded( ss DNA) current pulses with α-hemolysin( α-HL)single nanopore interface was investigated. Experimental results showed that the pulses positive charged protamine and negative ss DNA probes were both well observed with the established system,and both the pulse amplitude and duration of ss DNA were increased as a result of interaction with protamine. This study provides a way to improve the resolving power of current pulses based on molecular interactions.更多还原