【摘要】 蓝藻水华在全球范围频繁暴发,鱼腥藻(Anabaena)水华出现频度仅次于微囊藻(Microcystis)水华,其中的一些鱼腥藻种类能产生多样的藻毒素或异味物质,鱼腥藻水华去除技术的研发备受关注.本研究以从武汉市新洲区土壤中分离筛选到的一株细菌WP为材料,通过分析其生理生化特征及16S r DNA序列对其进行分类地位鉴定,考察了该菌株对真紧密鱼腥藻(Anabaena eucompacta)CHAB 1799的溶藻特性.结果表明,菌株WP与Lysinibacillus fusiformis NRS-350的同源性达到了99.6%,故鉴定WP属于Lysinibacillus属.菌株WP对真紧密鱼腥藻CHAB 1799发生溶藻作用的细菌阈值为6×10~3cfu/ml,其生理状态不会明显影响溶藻效果,且对不同生长时期的藻液均有较强的溶藻作用.该菌株的溶藻方式为间接溶藻.本研究结果丰富了溶藻细菌的资源库,也为治理鱼腥藻水华提供了更多的技术支持.更多还原

【Abstract】 Occurrence of cyanobacterial blooms is becoming a global environmental issue. Next to Microcystis bloom,Anabaena bloom is also the main bloom type with more diversity in production of cyanotoxins and odour substances,and removal of Anabaena blooms is also much concerned. One strain encoded as WP with strong algicidal activity against Anabaena eucompacta CHAB 1799 was isolated from a soil environment in Wuhan city. The 16 S rRNA gene sequence of the WP strain showed 99.6% similarity with that of Lysinibacillus fusiformis NRS-350. Based on morphological,physiological and molecular phylogenetic features,algicidal bacterium WP was identified as Lysinibacillus sp. Its algicidal characteristics on A. eucompacta was also tested. Lysinibacillus sp. WP lysed A. eucompacta CHAB 1799 at lowcell density( 6×10~3 cfu/ml),and lysis effects of the Lysinibacillus sp. WP on A. eucompacta were related to growth stages of A. eucompacta CHAB 1799 rather than the growth stages of the strain WP. The strain WP lysed algae by secreting metabolites. Such results in the present study increase the resource bank of algicidal bacteria,providing more potential to treat the newtype blooms such as A. eucompacta dominated bloom in China.更多还原