The flask-shaking batch fermentation conditions of mutant strain Brevibacterium flavum BM2610 were studied. The single factor for fermentation medium and culture conditions for producing L-isoleucine by strain BM2610 were optimized,respectively. The optimum carbon and nitrogen source of fermentation medium,the optimized initial pH,volume of broth and inoculation amount for fermentation medium were determined. The composition and ratio of fermentation medium were further optimized by response surface method....
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