【摘要】 在室温条件下,探讨不同饥饿时间对拟环纹豹蛛体内蛋白酶和脂肪酶活性的影响。结果表明:不同饥饿时间处理的拟环纹豹蛛雌、雄成蛛体内蛋白酶和脂肪酶活性均存在差异。在饥饿初期(雌蛛0～12h、雄蛛0～4h),饥饿处理对拟环纹豹蛛体内的蛋白酶活性起促进作用,雌、雄蛛的蛋白酶活性分别较未饥饿处理上升了36.8%和35.8%,差异显著;而后期起抑制作用,但差异不显著。拟环纹豹蛛雌、雄成蛛体内的脂肪酶活性,随着饥饿时间的延长均呈下降的趋势。在饥饿的初始阶段(0～4h),饥饿处理对脂肪酶活性的抑制作用较大,雌成蛛的脂肪酶活性较未饥饿处理的脂肪酶活性下降了39.6%,差异显著,雄成蛛的脂肪酶活性较未饥饿处理的脂肪酶活性下降了15.3%,差异不显著,而后期饥饿处理对拟环纹豹蛛雌、雄成蛛的脂肪酶活性影响均不明显。此外,拟环纹豹蛛雌成蛛的蛋白酶和脂肪酶活性在各相同饥饿处理时间均高于雄成蛛的蛋白酶和脂肪酶活性。更多还原

【Abstract】 This study was conducted to investigate the effect of starvation on major digestive enzymes(protease and lipase).The results showed that the effects of different starvation phases on digestive enzyme activities of male and female adult Pardosa pseudoannulata were different.In the early stage of starvation,the protease activities in female spiders(0-12h)and male spiders(0-4h)were promoted by starvation treatment,which increased by36.8% and 35.8%,respectively,compared with that in the 0hof starvation.However the protease activities in later stage were inhibited,but the difference was not significant.Lipase activities of female and male adult spiders decreased with the time of starvation.In the initial stage of starvation(0-4h),the starvation treatment had a great inhibitory effect.The lipase activity of female adult spiders was 39.6%lower than that treated with starvation for 0h,with a significant difference,while the lipase activity of male adult spiders was 15.3%lower than that treated with starvation for 0h,with no significant difference.However in the later stage of starvation treatment,little effect on the lipase activities of female and male adult spiders was observed.Treated for the same starvation time,the activities of protease and lipase of female spiders were significantly higher than those of male spiders.更多还原