【摘要】 为研究香椿组培快繁技术,对香椿组织培养的培养基配方进行筛选。在MS基本培养基中添加不同浓度的6-BA、NAA、GA3的正交试验结果表明,影响香椿芽增殖的主要因素是6-BA,且对芽增殖的影响达显著水平（P<0.05）,最佳质量浓度为1.0 mg·L^-1;对芽增殖影响次要因素是NAA,最佳质量浓度为0.1 mg·L^-1;GA3对芽增殖的影响最小,但对幼茎节间伸长生长有促进作用;芽增殖最佳培养基为MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+GA30.1 mg·L^-1,芽增殖倍数可达3.6倍。以MS、1/2 MS为基本培养基添加不同浓度的NAA、IBA进行的生根培养试验结果表明:MS+NAA 0.2mg·L^-1+IBA 0.2 mg·L^-1培养基生根效果最好,生根率可达25%。腐殖土作为香椿组培苗移栽基质,组培苗移栽成活率可达95%,苗木长势好。更多还原

【Abstract】 In order to study the tissue culture and rapid propagation of Toona sinensis,the medium formula for tissue culture of Toona sinensis was studied and screened.By adding different concentrations of 6-BA,NAA and GA3 in MS medium,the orthogonal experiment and variance analysis showed that the main factor affecting the proliferation of Toona sinensis was 6-BA,which had a significant effect on the bud proliferation（P < 0.05）,and the best concentration was 1.0 mg·L^-1;the secondary factor affecting bud proliferation was NAA,and the optimal concentration was 0.1 mg·L^-1;GA3 had the least effect on bud proliferation,but GA3 had a promoting effect on the internode elongation growth of young stems.The optimal bud culture medium combination was MS + 6-BA 1.0 mg·L^-1+ NAA 0.1 mg·L^-1+ GA30.1 mg·L^-1,and the multiplication ratio of shoots reached 3.6 times.MS and 1/2 MS medium supplemented with different concentrations of NAA and IBA to carry out rooting culture test,the results showed that the MS + NAA0.2 mg·L^-1+ IBA 0.2 mg·L^-1 formula combination medium had the best rooting effect,and the rooting rate was 25%.Taking humus soil as tissue culture seedlings transplanting toothache,the survival rate of the tissue culture seedlings transplanted was high up to 95%,and the seedlings grew well.更多还原