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不同苗龄牛樟叶绿素荧光差异性

Study of Chlorophyll Fluorescence Variations for Different Ages’ Cinnamomum kanehirae Hay. Seedling

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【作者】 杨杰; 郑蓉; 李建民; 温晓芸; 林永洲; 洪小龙; 郑维鹏;

【Author】 YANG Jie;ZHENG Rong;LI Jianmin;WEN Xiaoyun;LIN Yongzhou;HONG Xiaolong;ZHENG Weipeng;Fujian Academy of Forestry Sciences;The Key Laboratory of Southern Mountain Timber Forest Cultivation for the State Forestry Administration of the People’s Republic of China;The Key Laboratory of Forest Cultivation and Forest Products Processing and Utilization of Fujian Province;Zhangzhou Academy of Forestry Sciences;The state forest farm of Jiu longling in Zhangzhou;

【机构】 福建省林业科学研究院; 国家林业局南方山地用材林培育重点实验室; 福建省森林培育与林产品加工利用重点实验室; 漳州市林业科学研究所; 漳州市龙海九龙岭林场;

【摘要】 采用OS5p调制叶绿素荧光仪,对福建省龙海市九龙岭林场从台湾引种的不同苗龄的牛樟叶绿素荧光参数进行比较分析。结果表明:与1年生牛樟相比,3年生牛樟初始荧光(Fo)、最大荧光(Fm)、可变荧光(Fv)、最大荧光比率(Fv/Fm)、光合量子产量(ΦPSⅡ)、光化学猝灭系数(qP)及电子传送率(ETR)均显著升高(P<0.05),稳态荧光(Fs)、实际荧光(Fms)、非光化学猝灭系数(qN)及光能非光化学耗散(N)均显著降低(P<0.05)。与温室栽培相比,同龄露天栽培牛樟幼苗qN、D、ΦPSⅡ和ETR显著升高(P<0.05)。与本地香樟相比,同龄牛樟Fm、Fv、Fv/Fm及Fv/Fo显著升高(P<0.05),表明牛樟潜在最大光合能力较高,PSⅡ潜在活性较高,应对环境胁迫的能力也较强;而本地香樟对环境的适应性较强,实际光合量子产量也较高。叶绿素荧光参数间相关性分析结果表明,Fm与Fv(R2=0.990),ΦPSⅡ与ETR(R2=0.996),P与ETR(R2=0.923)及ΦPSⅡ与P(R2=0.935)之间具有极显著正相关关系;ΦPSⅡ与D(R2=0.993),ETR与D(R2=0.999)之间具有极显著负相关关系。

【Abstract】 As an ″inner probe″,chlorophyll fluorescence(Cf) has been used for the studies of plants photosynthetic characteristics exploring. In this study,1 3-years-old Cinnamomum kanehirae Hay.(C. kanehirae Hay.) were selected as experimental materials to discuss the variation of Cf under different environmental conditions. Under the same environmental conditions,Cf parameters were determined by OS5 p chlorophyll fluorometer. The results showed that 3-year-old C. kanehirae Hay. performed a significant increase(P < 0. 05) in dark-adapted minimum fluorescence(Fo),dark-adapted maximum fluorescence(Fm),variable fluorescence(Fv),the maximum potential quantum efficiency of photosystem Ⅱ(Fv/Fm),photochemical efficiency(ΦPSⅡ),photochemical quenching coefficient(q P) and apparent electron transfer rate(ETR) when compared with the 1-year-old C. kanehirae Hay.,and showed a significant decrease(P < 0. 05) in steady-state Cf(Fs),light-adapted Cf(Fms) and non-photochemical quenching coefficient(qN)on the contrary. Compared with the C. kanehirae Hay. which cultivated in artificially controlled greenhouse,the 1-year-old C. kanehirae Hay. which cultivated outside field has a significant increase(P < 0. 05) for qP,D,ΦPSⅡand ETR,which indicate that appropriate environmental stress is a beneficial factor to the Pn improving and light energy efficiency promoting. At the same time,compared with the same age of C. camphora,the Fm,Fv,Fv/Fmand Fv/Foof C. kanehirae Hay. were significantly increased. For C. kanehirae Hay. and C. camphora,there was significant linear correlation between Fmand Fv(R2= 0. 990),and between ΦPSⅡand ETR(R2= 0. 996),and between ETR and energy photochemical quenching(P)(R2= 0. 923),and between ΦPSⅡand P(R2=0. 935). And there was significant negative linear correlation between ΦPSⅡand energy antenna heat dissipation(D)(R2= 0. 993),and between ETR and D(R2= 0. 999).

【基金】 国家林业局948引进项目(2013-4-33)
  • 【文献出处】 福建林业科技 ,Journal of Fujian Forestry Science and Technology , 编辑部邮箱 ,2018年01期
  • 【分类号】S792.23
  • 【被引频次】6
  • 【下载频次】144
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